Type
MonoclonalAntibody
Applications
Westernblotting,Immunoprecipitation
Hosts
Mouse
Isotype
IgG1
Clone
E19
Preparation
WB(withECL):1:1000–10,000 dilution(incubateforonehouratroomtemperature)Forbestresultswithotherassays(e.g.:IP,etc),pleasedetermineoptimalworkingdilutionbytitration test.
PurificationMethod
ProteinA affinitychromatographyfrommouseascites fluid.
Formulation
1 mg/mLin10 mMPBS,pH7.2,with0.05%NaN3. Storeat –20°Cforupto2 years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze-thawcycles.Storealiquotsat4°Cfor1–2 weeks.Storage/Expiration