Type
MonoclonalAntibody
Applications
Westernblotting,ELISA,Dot,IS,Immunoprecipitation
Hosts
V4RR
Isotype
IgG1
Clone
E10
Preparation
WB(withECL):1:1000–3000dilution(incubatefor1 houratroomtemperature)IS:1:500–2000dilutionForbestresultswithotherassays(e.g.:Dot,ELISA,IP,etc),pleasedetermineoptimalworkingdilutionbytitration test.
PurificationMethod
ProteinA affinitychromatographyfromculturesupernatantofhybridomacellsgrowninabioreactor.
Formulation
1 mg/mLin10 mMPBS,pH7.2,with0.05%NaN3. Storeat –20°Cforupto2 years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze-thawcycles.Storealiquotsat4°Cfor1–2 weeks.Storage/Expiration