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Bpsbioscience/GCN5 Fluorogenic Assay Kit/50091/96 reactions
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Description:TheFluorogenicGCN5AssayKitisahomogeneous,fluorogenicassaydesignedtoscreenforinhibitorsofGCN5(alsoknownasKAT2A).TheFluorogenicGCN5AssayKitisbasedonthetransferofanacetylgroupfromacetylCoAtoapeptidesubstrate.AfterincubationwithacetylCoAandthesubstrate,theGCN5enzymegeneratesacetylatedH3peptideandCoASH.Notime-consumingwashingstepsarerequired.ThethiolgroupsofCoASHcanbedetectedwithfluorogenicreagentatexcitation=360nmandemission=460nm.UniProtQ92830Synonym(s):HAT,histoneacetyltransferase,GCN5,KAT2A,GCNL2,PCAF-βSuppliedAs:TheFluorogenicGCN5AssayKitcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentHATactivitymeasurements.Inaddition,thekitincludespurifiedGCN5foruseasapositivecontrol.Contraindications:DTT,β-mercaptoethanol,orotherreducingreagents,DMSO>1%,strongacidsorbases,ionicdetergents,highsaltFormat:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.Reference(s):Trievel,R.C.,etal.(2000).Anal.Biochem.287(2):319-28.ApplicationReference(s):1.Dual-ModeHDACProdrugforCovalentModificationandSubsequentInhibitorRelease(2015)2.Acomparativestudyofmetalandplastic(Karman)cannulaeforfirsttrimesterabortionbysuctioncurettage.(2015)Warning(s):Avoidfreeze/thawcycles.ScientificCategory:AcetyltransferaseProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。

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