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Bpsbioscience/14-3-3 eta Protein, GST-tag (Human)/79031/20 μg
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Species:HumanHostSpecies:E.coliMW:56kDaGenbank#:NM_003405Tag(s):N-terminalGST-taga.a:FulllengthBackground:14-3-3eta(alsoknownastyrosine3-monooxygenase/tryptophan5-monooxygenaseactivationprotein,etapolypeptide)playsakeyroleintissuedifferentiationinneuraldevelopmentandinearlymesenchyme(1).The14-3-3etaproteinbelongstofamilyofacidicproteinspresentexclusivelyinthebrainandisbelievedtohaveafunctioninmonoaminebiosynthesis.14-3-3etaproteinregulateshumantyrosinehydroxylasetype1(hTH1)stABIlitybyactingontheN-terminus.14-3-3etacanbindtoparkin,whichencodesanE3ubiquitin-proteinligase,inthelinkerregionandnegativelyregulateitsubiquitinligaseactivity(2).Description:Human14-3-3etaProtein,GenBankAccessionNo.NM_003405,fulllength,withN-terminalGST-tag,expressedinanE.coliexpressionsystem.MW=56kDa.Synonym(s):YWHAH,YWHA1Formulation:50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.Storage/Stability:Storeproductat–70°C.Foroptimalstorage,aliquottargetintosmallerquantitiesaftercentrifugationandstoreatrecommendedtemperature.Formostfavorableperformance,avoidrepeatedhandlingandmultiplefreeze/thawcycles.Application(s):WesternblotReference(s):1.Ichimura,T.etal:MolecularcloningofCDNAcodingforbrain-specific14-3-3protein,aproteinkinase-dependentactivatoroftyrosineandtryptophanhydroxylases.Proc.Natl.Acad.Sci.USA.1988;85(19):7084-8.2.Sato,S.etal:14-3-3etaisanovelregulatorofparkinubiquitinligase.EMBOJ.2006Jan11;25(1):211-21.ScientificCategory:KinaseProductType:ProteinDatashownislot-specific.Contactusforspecificinformationonotherlots.
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