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Bpsbioscience/ICK, GST-tag/40112/10 µg
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产品说明
Species: Human
Host Species: Sf9 insect cells
MW: 98 kDa
Genbank #: NM_014920
Tag(s): N-terminal GST-tag
a.a: full length
Background: ICK or intestinal cell kinase belongs to a very extensive family of proteins that share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. ICK encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases (1). ICK localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. ICK purified from transfected human embryonic kidney cells undergoes autophosphorylation and phosphorylates a test protein. ICK plays a key role in development of central nervous, skeletal, and endocrine systems (2).
Description:Recombinant human intestinal cell kinase (ICK), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Synonym(s): ECO; MRK; KIAA0936; LCK2; MGC46090
Purity: ≥75%
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Togawa, K. et.al: Intestinal cell kinase (ICK) localizes to the crypt region and requires a dual phosphorylation site found in map kinases. J. Cell. Physiol. 183: 129-139, 2000.2. Lahiry, P. et.al: A multiplex human syndrome implicates a key role for intestinal cell kinase in development of central nervous, skeletal, and endocrine systems.
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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