首页 > BPS Bioscience > Bpsbioscience/P300 (KAT3B) Fluorogenic Assay Kit/50092/96 reactions

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Bpsbioscience/P300 (KAT3B) Fluorogenic Assay Kit/50092/96 reactions

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50092

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Description:TheFluorogenicp300AssayKitisahomogeneous,fluorogenicassaydesignedtoscreenforinhibitorsofp300(alsoknownasKAT3B).TheFluorogenicp300AssayKitisbasedonthetransferofanacetylgroupfromacetylCoAtoapeptidesubstrate.AfterincubationwithacetylCoAandthesubstrate,theP300enzymegeneratesacetylatedH3peptideandCoASH.Notime-consumingwashingstepsarerequired.ThethiolgroupsofCoASHcanbedetectedwithfluorogenicreagentatexcitation=360nmandemission=460nm.UniProtQ09472Synonym(s):HAT,histoneacetyltransferase,p300,HumanE1Abindingproteinp300,EP300,KAT3B,RSTS2SuppliedAs:TheFluorogenicp300AssayKitcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentHATactivitymeasurements.Inaddition,thekitincludespurifiedp300foruseasapositivecontrol.Contraindications:DTT,β-mercaptoethanol,orotherreducingreagents,DMSO>1%,strongacidsorbases,ionicdetergents,highsaltFormat:Components: Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.Reference(s):Trievel,R.C.,etal.(2000).Anal.Biochem.287(2):319-28.Warning(s):Avoidfreeze/thawcycles.ScientificCategory:AcetyltransferaseProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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