首页 > BPS Bioscience > Bpsbioscience/P300 Homogeneous Assay Kit/50078/384 reactions

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Bpsbioscience/P300 Homogeneous Assay Kit/50078/384 reactions

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50078

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4000-520-616

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Background:TheadenovirusE1A-associatedcellularp300transcriptionalco-activatorproteinfunctionsasahistoneacetyltransferasethatregulatestranscriptionviachromatinremodelingandisimportantintheprocessesofcellproliferationanddifferentiation.p300(alsoknownasKAT3B)acetylatesallfourcorehistonesinnucleosomes,aswellasnon-histonetargets.p300mediatescAMP-generegulationbybindingspecificallytophosphorylatedCREBprotein.p300hasalsobeenidentifiedasaco-activatorofHIF1A(hypoxia-inducIBLefactor1alpha),whichplaysaroleinthestimulationofhypoxia-inducedgenessuchasVEGF.DefectsinthisgeneareacauseofRubinstein-TaybisyndromeanditsdysfunctionislinkedtoanumberofhumandiseasesincludingParkinson’s,Huntington’s,andAlzheimer’sdiseases.p300mayalsoplayaroleinepithelialcancer.Description:Thep300HomogeneousAssayKitcontainsanAlphaLISA®assaydesignedforscreeninginhibitorsofp300inaconvenient384-wellformat.Thisefficientmethodrequiresnotime-consumingwashingsteps.Onlyafewsimplestepsonamicrotiterplateareneeded.TheassayisbasedontheenzymatictransferofanacetylgroupfromacetylCoAtoaspecificlysineresiduewithinabiotinylatedpeptidesubstrate.Afterthep300reaction,thesampleisincubatedwithacombinationofanantibodythatspecificallybindstheacetylatedpeptideandacceptorbeadsthatbindtheantibody.Thisisfollowedbyincubationwithstreptavidin-labeleddonorbeadsandreADIngoftheAlpha-counts.UniProtQ09472Synonym(s):EP300,KAT3B,RSTS2,E1AbindingproteinSuppliedAs:Thep300HomogeneousAssayKitcomesinaconvenientAlphaLISA®format,withbiotinylatedhistonesubstrate,AcetylCoA,,primaryantibody,assaybuffers,andpurifiedp300for384enzymereactions.Contraindications:AvoidDMSOconcentrationsabove0.5%.AvoidgreenandbluedyesthatabsorblightintheAlphascreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphascreen®assays.Format:Storage/StABIlity:Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):GreatforstudyingenzymeactivityandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.Reference(s):Trievel,R.C.,etal.(2000).Anal.Biochem.287(2):319-28.Notes:AlphaScreen®andAlphaLISA®areregisteredtrademarksofPerkinelmer,Inc.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AcetyltransferaseProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。