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Bpsbioscience/Anti–3–mC polyclonal antibody/25200/50 µg

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25200

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HostSpecies:RabbitClonality:polyclonalBackground:3–methylcytosinearisesbyerroneousmethylationofDNAthroughspontaneousexposuretoendogenousS–adenosylmethionine(SAM).SincetheN3–positionincytosineisprotectedindoublestrandedDNA,thisphenomenonmainlyoccursinsinglestrandedDNA.Theresulting3–mCistoxicandmutagenicandincreasedlevelsof3–mCseemtoimpaircellproliferation.Therefore,ithastoberepairedbythecell.Thisisestablishedbyoxidativedemethylation,whichiscatalyzedbytheAlkBprotein.Description:Polyclonalantibodyraisedinrabbitagainst3–methylcytosine(3–mC)conjugatedtoBSA.AssayConditions:Dotblotanalysisusingtheantibodydirectedagainst3-mCTodemonstratethespecificityoftheantibodyagainst3-mC(cat.No.25200),aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedbases.150and50pmoloftherespectiveoligo’swerespottedonthemembrane.Theantibodywasdiluted1:400inPBS-Tcontaining10%skimmedmilkand1%BSA.Figure1showsahighspecificityoftheantibodyfortheoligonucleotidewiththe3-methylcytosinemodification.Concentration:50µg/28µlFormulation:PBScontaining0.05%azideand0.05%ProClin300.SpeciesReactivity:Human,mouse,broadrangeCrossReactivity:Doesnotcross–reactwith5–methylcytosine,5–hydroxymethylcytosine,or5–carboxylcytosine.Purification:ProteinGpurifiedImmunogen:syntheticpeptideFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):DB(1:400)Notes:Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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