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Bpsbioscience/Anti–AML1–ETO polyclonal antibody/25208/100 µl

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HostSpecies:RabbitClonality:polyclonalBackground:TheAML1(RUNX1)(UniProtKB/Swiss–ProtentryQ01196)–ETO(RUNX1T1)(UniProtKB/Swiss–ProtentryQ06455)fusionproteinarisesduetoatranslocationbetweenchromosome8and22(t(8;21)(q22;q22)).Thistranslocationisoneofthemostfrequentkaryotypicabnormalitiesobservedinacutemyeloidleukaemia.Itproducesachimericalgenemadeupofthe5’–regionofAML1andthe3’–regionofETO.Thechimericalproteinisthoughttoassociatewiththenuclearcorepressor/histonedeacetylasecomplextoblockhematopoieticdifferentiation.Description:PolyclonalantibodyraisedinrabbitagainsttheAML1–ETOfusionproteinusingaKLH–conjugatedsyntheticpeptide.Synonym(s):RUNX1–RUNX1T1fusionproteinAssayConditions:ChIPresultsobtainedwiththeantibodydirectedagainstAML1-ETOChIPassayswereperformedusingKasumi-1cells,theantibodyagainstAML1-ETO(Cat.#25208)andoptimizedprimerpairsforqPCR.Shearedchromatinfrom1.25millioncellsand4µlofantibodywereusedperChIPexperiment.QPCRwasperformedusingprimersspecificfortheFUT7,NFE2andOGG1genes.Figure1showstheoccupancy,calculatedastheratio+control/backgroundforwhichthepromoteroftheH2Bgenewasused.ChIP-seqresultsobtainedwiththeantibodydirectedagainstAML1-ETOChIPwasperformedasdescribedabove.TheimmunoprecipitatedDNAof6ChIPassayswaspooledandanalyzedwithanIlluminaGenomeAnalyzer.Librarypreparation,clustergenerationandsequencingwereperformedaccordingtothemanufacturer"sinstructions.The32bptagswerealignedtothehumanreferencegenome(hg18)usingtheELANDalgorithm.Figure2showstheresultsofthecompletechromosome3andthreegenomicregionssurroundingtheOGG1,FUT7andNFE2genes,respectively.ThepositionofthePCRampliconisindicatedwithanarrow.DeterminationoftheantibodytiterTodeterminethetiteroftheantibody,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstAML1-ETO(Cat.#25208).Theplateswerecoatedwiththepeptidesusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure3),thetiteroftheantibodywasestimatedtobe1:32,750.WesternblotanalysisusingtheantibodydirectedagainstAML1-ETONuclearextractsofSKNO-1cells(15µg)wereanalyzedbyWesternblotusingtheantibodyagainstAML1-ETO(Cat.#25208)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.TheMarker(inkDa)isshownontheleft,thepositionoftheproteinofinterestisindicatedontheright. Concentration:100µlFormulation:Wholeantiserumfromrabbitcontaining0.05%azideSpeciesReactivity:HumanPurification:WholeantiserumImmunogen:syntheticpeptideFormat:aqueoussolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ChIP/ChIP–seq(4µl/ChIP)ELISA(1:500)WB(1:1000)Notes:TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIPWarning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。