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Bpsbioscience/Anti–BRD2 polyclonal antibody/25211/100 µl
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HostSpecies:RabbitClonality:polyclonalBackground:Brd2(UniProtKB/Swiss–ProtentryP25440),amitogen–activatedkinaselocalizedtothenucleus,isaputativetranscriptionalregulatorwhichinteractswithE2F1andwithhistoneH4acetylatedatLys–13.Itisexpressedduringdevelopmentandmaybeinvolvedingrowthcontrol.Brd2alsomayplayaroleinspermatogenesisorfolliculogenesisandmaybeinvolvedinsometypesofleukemiaandinjuvenilemyoclonicepilepsy.Description:PolyclonalantibodyraisedinrabbitagainstmouseBrd2(bromodomaincontaining2),usingaKLHconjugatedsyntheticpeptidecontaininganaminoacidsequencefromthecentralpartoftheproteinAssayConditions:DeterminationofthetiterTodeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseBrd2(Cat.#25211).Thewellswerecoatedwiththepeptideusedforimmunisationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:5,000.WesternblotanalysisusingtheantibodydirectedagainstBrd2Westernblotwasperformedonwholecelllysatesfrommousefibroblasts(NIH3T3)andembryonicstemcells(E14Tg2a)withtheantibodyagainstmouseBrd2(Cat.#25211),diluted1:1,000inBSA/PBS-Tween.ThemolecularweightMarker(M,inkDa)isshownontheleft;thelocationoftheproteinofinterest(predictedsize:88kDa)isindicatedontheright. Concentration:100µlFormulation:Wholeantiserumfromrabbitcontaining0.05%azideSpeciesReactivity:MousePurification:WholeserumImmunogen:syntheticpeptideFormat:aqueoussolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ELISA(1:100)WB(1:1000)Notes:Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。

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