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Bpsbioscience/Anti–CIITA polyclonal antibody/25214/50 µg

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25214

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HostSpecies:RabbitClonality:polyclonalBackground:CIITA(UniProtKB/Swiss–ProtentryP33076)isessentialfortranscriptionalactivityoftheHLAclassIIpromoter.Itmayactinacoactivator–likefashionthroughprotein–proteininteractionsbycontactingfactorsbindingtotheproximalMHCclassIIpromoter,ortoelementsofthetranscriptionmachinery.AlternativelyCIITAmayactivateHLAclassIItranscriptionbymodifyingproteinsthatbindtotheMHCclassIIpromoter.DefectsinCIITAcausebarelymphocytesyndrometypeII(BLSII);alsoknownashereditaryMHCclassIIdeficiencyorHLAclassII–deficientcombinedimmunodeficiency.Description:PolyclonalantibodyraisedinrabbitagainsthumanCIITAprotein(MHCclassIItransactivator),usingarecombinantprotein.Synonym(s):C2TA,MHC2TAAssayConditions:ChIPresultsobtainedwiththeantibodydirectedagainstCIITAChIPassayswereperformedusingNALMcells(acelllinederivedfromhumanpre-Bleukemia),theantibodyagainstCIITA(Cat.#25214)andoptimizedprimersetsforPCR.Shearedchromatinfrom2millioncellsandrespectively5and7µgofantibodywereusedperChIPexperiment.Ananti-FLAGantibody(lane2)wasusedasnegativeIPcontrol.Figure1showstheresultoftheend-pointPCRwithprimersforHLA_DRA,usedaspositivecontrol(lowerpanel)andforHLA_DOB,usedanegativePCRcontrol(upperpanel). Concentration:50µg/50µlFormulation:PBScontaining0.05%azideand0.05%ProClin300.SpeciesReactivity:HumanPurification:AffinitypurifiedImmunogen:recombinantproteinFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ChIP(5–7µg/IP)Notes:TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–10µgperIPWarning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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