首页 > BPS Bioscience > Bpsbioscience/Anti–Cre polyclonal antibody/25215/100 µl

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Bpsbioscience/Anti–Cre polyclonal antibody/25215/100 µl

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25215

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4000-520-616

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HostSpecies:RabbitClonality:polyclonalBackground:CrerecombinaseoriginatesfrombacteriophageP1andrecognizesloxPsitesinthegenome.ItcatalyzesrecombinationbetweentwoloxPsites,therebyexcisingtheinterveningDNAsequence.CrerecombinaseisusedasatoolforthegenerationoftransgenicanimalsDescription:PolyclonalantibodyraisedinrabbitagainstCrerecombinaseusingaKLH–conjugatedsyntheticpeptidelocatedattheC–terminalpartoftheproteinSynonym(s):CrerecombinaseAssayConditions:DeterminationoftheantibodytiterTodeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstCre(cat.No.25215)inantigencoatedwells.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:2,900.WesternblotanalysisusingtheantibodydirectedagainstCre(1)Westernblotwasperformedonwholecelllysatesfromuntransfected293cells(lane1),or293cellstransfectedwithCre(lane2),Dre(lane3)orFlp(lane4)withtheantibodyagainstCre(cat.No.25215),diluted1:500inBSA/PBS-Tween.ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterest(expectedsize:38kDa)isindicatedontheright. Concentration:100µlFormulation:Wholeantiserumfromrabbitcontaining0.05%azideSpeciesReactivity:NAPurification:WholeserumImmunogen:syntheticpeptideFormat:aqueoussolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ELISA(1:100)WB(1:500)Notes:Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。