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Bpsbioscience/Anti–DNMT3A polyclonal antibody/25218/50 µg

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25218

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4000-520-616

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HostSpecies:RabbitClonality:polyclonalBackground:DNMT3A(UniProtKB/Swiss–ProtentryQ9Y6K1)catalysesthegenomewidedenovomethylationofCpGresidues.DNAmethylationonCpGresiduesbyDNMT3Aregulatesgeneexpressionandisessentialfordevelopment.DNMT3Aisstronglyexpressedinembryonicstemcells,butlowinadultsomaticcells.DNAmethylationiscoordinatedwithmethylationofhistones.DNMT3AbindstoSETDB1andHDAC1,andisinvolvedintherepressionoftranscriptionfrompromoterscontaininganE2Fbindingsite.Description:PolyclonalantibodyraisedinrabbitagainsthumanDNMT3A,(DNAmethyltransferase3A),usingaKLH–conjugatedsyntheticpeptidecorrespondingtoaminoacids92–106.AssayConditions:DeterminationoftheantibodytiterTodeterminethetiteroftheantibody,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainsthumanDNMT3A(Cat.#25218),crudeserumandflow-throughinantigencoatedwells.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:2,000.WesternblotandImmunoprecipitationusingtheantibodydirectedagainstDNMT3AHumanembryonickidneycells(HEK293T)weretransientlytransfectedwithanexpressionvectorforGAL4-taggedDNMT3A.Wholecellextractswereimmunoprecipitatedwith2µgofantibodyagainstDNMT3A(Cat.#25218).ThepresenceofGAL4-DNMT3Ainthenon-treatedcellextractsandintheimmunoprecipitateswasdemonstratedbyWesternblotanalysiswithanti-GAL4antibody(lane1and2respectively).Alternatively,GAL4-DNMT3AwasimmunoprecipitatedandWesternblotanalysiswasperformedwiththeDNMT3Aantibody(diluted1:2,500)(lane3).Concentration:50µg/54µlFormulation:PBScontaining0.05%azideand0.05%ProClin300SpeciesReactivity:HumanPurification:AffinitypurifiedImmunogen:syntheticpeptideFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ELISA(1:100)WB(1:2500)IP(2µg/IP)Notes:Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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