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Bpsbioscience/Anti–DNMT3B polyclonal antibody/25219/50 µg

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25219

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4000-520-616

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HostSpecies:RabbitClonality:polyclonalBackground:DNMT3B(UniProtKB/Swiss–ProtentryQ9UBC3)catalysesthegenomewidedenovomethylationofCpGresidues,whichregulatesgeneexpression.DNMT3Bisessentialfordevelopment.DNAmethylationonCpGresiduesiscoordinatedwithmethylationofhistones.SixdifferentisoformsofDNMT3B,producedbyalternativesplicing,existalthoughisoforms4and5maynotbefunctionalduetotheabsenceoftwoconservedmethyltransferasemotifs.Description:PolyclonalantibodyraisedinrabbitagainstmouseDNMT3B(DNAmethyltransferase3B),using3KLHconjugatedsyntheticpeptidescontainingsequencesfromdifferentpartsoftheprotein.AssayConditions:DeterminationoftheantibodytiterTodeterminethetiteroftheantibody,anELISAwasperformedusingaserialdilutionofantibodydirectedagainstDNMT3B(Cat.No.25219).Theplateswerecoatedwiththepeptidesusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:220,000.WesternblotanalysisusingtheantibodydirectedagainstDNMT3BWholecellextracts(25μg)fromHeLacellswereanalysedbyWesternblotusingtheantibodyagainstDNMT3B(Cat.No.25219)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterestisindicatedontheright;theMarker(inkDa)isshownontheleft.ImmunofluorescenceusingtheantibodydirectedagainstDNMT3BHumanHeLacellswerestainedwiththeantibodyagainstDNMT3B(Cat.No.25219)andwithDAPI.Cellswerefixedwith4%formaldehydefor10minutesandblockedwithPBS/TX-100containing5%normalgoatserumand1%BSA.ThecellswereimmunofluorescentlylabelledwiththeDNMT3Bantibody(left)diluted1:1,000inblockingsolutionfollowedbyananti-rabbitantibodyconjugatedtoAlexa488.ThemiddlepanelshowsstainingofthenucleiwithDAPI.Amergeofthetwostainingsisshownontheright.Concentration:50µg/16µlFormulation:PBScontaining0.05%azideand0.05%ProClin300SpeciesReactivity:Human,mousePurification:AffinitypurifiedImmunogen:syntheticpeptideFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ELISA(1:10,000)WB(1:1000)IF(1:1000)Notes:Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.Warning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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