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Bpsbioscience/Anti–EZH2 monoclonal antibody/25222/50 µg

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25222

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4000-520-616

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HostSpecies:MouseClonality:monoclonalIsotype:IgG1Background:EZH2(UniProt/Swiss–ProtentryQ15910)isahistone–lysinemethyltransferasewhichmethylatesLys–9andLys–27ofhistoneH3,leADIngtotranscriptionalrepression.Itisamemberofthepolycombgroup(PcG)familywhichformmultimericproteincomplexesandareinvolvedinmaintainingthetranscriptionalrepressivestateofgenesoversuccessivecellgenerations.TheEZH2activityisdependentontheassociationwithothercomponentsofthePRC2complex(EED,SUZ12/JJAZ1,RBBP4andRBBP7).EZH2mayplayaroleinthehematopoieticandcentralnervoussystems.Over–expressionofEZH2isobservedduringadvancedstagesofprostatecancerandbreastcancer.Description:MonoclonalantibodyraisedinmouseagainstthecentralpartofthehumanEZH2protein(Enhancerofzestehomolog2)Synonym(s):ENX–1,KMT6AssayConditions:ChIPresultsobtainedwiththemonoclonalantibodydirectedagainstEZH2ChIPassayswereperformedusing2.5µgofthemonoclonalantibodyagainstEZH2(Cat.#25222)onshearedchromatinfrom5millionE14mouseembryonicstemandfromSUZ12-/-cells,usedasanegativecontrol.IgGwasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersspecificfortheHoxA10geneandfortheTgenewhichencodestheBrachyurytranscriptionfactor.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis). Concentration:50µg/50µlFormulation:PBScontaining0.05%azideSpeciesReactivity:Human,mousePurification:ProteinGpurifiedImmunogen:recombinantproteinFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ChIP(2.5µg/ChIP)Notes:TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIPWarning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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