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Bpsbioscience/Anti–EzH2 polyclonal antibody/25223/50 µg

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25223

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HostSpecies:RabbitClonality:polyclonalBackground:EZH2(UniProt/Swiss–ProtentryQ15910)isahistone–lysinemethyltransferasewhichmethylatesLys–9andLys–27ofhistoneH3,leADIngtotranscriptionalrepression.Itisamemberofthepolycombgroup(PcG)familywhichformmultimericproteincomplexesandareinvolvedinmaintainingthetranscriptionalrepressivestateofgenesoversuccessivecellgenerations.TheEZH2activityisdependentontheassociationwithothercomponentsofthePRC2complex(EED,SUZ12/JJAZ1,RBBP4andRBBP7).EZH2mayplayaroleinthehematopoieticandcentralnervoussystems.Over–expressionofEZH2isobservedduringadvancedstagesofprostatecancerandbreastcancer.Description:PolyclonalantibodyraisedinrabbitagainsttheN–terminus(aa1–343)ofthemouseEZH2protein(Enhancerofzestehomolog2).Synonym(s):ENX–1,KMT6AssayConditions:ChIPresultsobtainedwiththeantibodydirectedagainstEZH2ChIPassayswereperformedusingHeLacells,theantibodyagainstEZH2(Cat.#25223)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedusingshearedchromatinfrom1millioncells.Atitrationoftheantibodyconsistingof0.5,1,2.5and5µgperChIPexperimentwasanalyzed.IgG(2µg/IP)wasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersforMYT1,usedasapositivecontroltarget,andforthepromoteroftheactiveGAPDHgene,usedasanegativecontrol.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).WesternblotanalysisusingtheantibodydirectedagainstEZH2NuclearextractsofHeLacells(40µg)wereanalyzedbyWesternblotusingtheantibodyagainstEZH2(Cat.#25223)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterest(expectedsize85kDa)isindicatedontheright;theMarker(inkDa)isshownontheleft. Concentration:50µg/50µlFormulation:PBScontaining0.05%azideand0.05%ProClin300SpeciesReactivity:Human,mousePurification:ProteinGpurifiedImmunogen:recombinantproteinFormat:AqueousbuffersolutionStorage/StABIlity:Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.Application(s):ChIP(2.5µg/ChIP)WB(1:1000)Immunochemistry(1:100–1:500)Notes:TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIPWarning(s):Avoidfreeze/thawcyclesScientificCategory:AntibodiesProductType:AntibodyDatashownislot-specific.Contactusforspecificinformationonotherlots.

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