首页 > BPS Bioscience > Bpsbioscience/TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80579/384 reactions

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Bpsbioscience/TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80579/384 reactions

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80579

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Background:TNKS1catalyzestheNAD-dependentadditionofpoly(ADP-ribose)tothesubstrateproteins.Description:TheTNKS1HistoneRibosylationAssayKit(Biotin-labeledNAD+)isdesignedtomeasureTankyrase1(TNKS1)activityforscreeningandprofilingapplications.ThekeytotheTNKS1HistoneRibosylationAssay(Biotin-labeledNAD+)isthebiotinylatedsubstrate.Withthiskit,onlythreesimplestepsarerequiredforTNKS1reactions.First,histoneproteinsarecoatedona384-wellplate.Next,thebiotinylatedTNKS1substrateisincubatedwithanassaybufferthatcontainstheTNKS1enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanbemeasuredusingachemiluminescencereader.Synonym(s):Tankyrase1,TNKS1,poly(ADP-ribose)polymerase5a,poly(ADP-ribosyl)transferase5a,PARP5aSuppliedAs:TheTNKS1assaykitcomesinaconvenient384-wellformat,withpurifiedTNKS1enzyme,histonemixture,andPARPassaybufferfor384enzymereactions.Contraindications:DMSO>1%,strongacidsorbases,ionicdetergents,highsaltFormat:COMPONENTS:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.Reference(s):Brown,J.A.,Marala,R.B.J.Pharmacol.Toxicol.Methods200247:137-41.ApplicationReference(s):Scaffoldhoppingapproachontheroutetoselectivetankyraseinhibitors(2014)Warning(s):Avoidfreeze/thawcycles.ScientificCategory:PARPProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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