首页 > BPS Bioscience > Bpsbioscience/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80578/96 reactions

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Bpsbioscience/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80578/96 reactions

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80578

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BPS Bioscience

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Background:TNKS2catalyzestheNAD-dependentadditionofpoly(ADP-ribose)tothesubstrateproteins.Description:TheTNKS2HistoneRibosylationAssayKit(Biotin-labeledNAD+)isdesignedtomeasureTankyrase2(TNKS2)activityforscreeningandprofilingapplications.ThekeytotheTNKS2HistoneRibosylationAssayisthebiotinylatedNAD+substrate.Withthiskit,onlythreesimplestepsarerequiredforTNKS2reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thebiotinylatedNAD+substrateisincubatedwithanassaybufferthatcontainstheTNKS2enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanbemeasuredusingachemiluminescencereader.Synonym(s):Tankyrase2,TNKS2,poly(ADP-ribose)polymerase5,poly(ADP-ribosyl)transferase,PARP5b,PARP5cSuppliedAs:TheTNKS2assaykitcomesinaconvenient96-wellformat,withpurifiedTNKS2enzyme,histonemixture,andPARPassaybufferfor100enzymereactions.Contraindications:DMSO>1%,strongacidsorbases,ionicdetergents,highsaltFormat:COMPONENTS:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.Reference(s):Brown,J.A.,Marala,R.B.J.Pharmacol.Toxicol.Methods200247:137-41.ApplicationReference(s):DisruptionofWnt/β-CateninSignalingandTelomericShorteningAreInextricableConsequencesofTankyraseInhibitioninHumanCells(2015)Warning(s):Avoidfreeze/thawcycles.ScientificCategory:PARPProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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