首页 > BPS Bioscience > Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg

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Bpsbioscience/B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™/71114/50 µg

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71114

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Species:HumanHostSpecies:HEK293MW:52.5kDaGenbank#:NM_005191Tag(s):C-terminalFcfusion(HumanIgG1),C-terminalAvi-tag™a.a:35-242Description:HumansecretedB7-1-Fcfusionprotein,alsoknownasCD80,withC-terminalAvi-tag™.GenBankAccessionNo.NM_005191,a.a35-242expressedinaHEK293cellexpressionsystemandenzymaticallybiotin-labeledusingAvi-tag™technology.MW=52.5kDa(monomer).ThisproteinrunsatahigherM.W.bySDS-PAGEduetoglycosylation.UniProtP33681Synonym(s):CTLA-4Counter-ReceptorB7.1AssayConditions:ReactiondoneaccordingtoCTLA4:B7-1[biotin]assaykit(Catalog#72009)protocol.BufferisPBS+0.1%BSA.BlockingbufferisSuperblock+0.05%Tween-20.CoatproteinwasaddedONat4deg100ng/well.Variousconcentrationsofbiotinylatedligand(0-8ng/ul,0.5ng/ulistherecommendedkitcondition)addedfor2hatRT.Strep-HRPaddedfor1hatRT.Formulation:40mMTris,pH8.0,110mMNaCl,2.2mMKCl,and20%glycerol.Format:AqueousbuffersolutionStorage/StABIlity:Atleast6monthsat–80°C.Application(s):Usefulforthestudyofprotein-proteininteractionandinvitroandinvivoproteinfunctionstudies.Reference(s):1.Byrum,J.N.,VanKomen,J.S.,Rodgers,W.,J.Immunol.2013Sep15;191(6):3073-3081.2.Legat,A.,etal.,FrontImmunol.2013Dec19;4:455.Notes:HiP™indicatesahighpurityproteinwith≥90%purityasmeasuredbygelfiltration.Warning(s):Avoidfreeze/thawcycles.Proteinmaybedilutedto≥100μg/mlinPBS+glycerolandstoredat-80°C.ScientificCategory:ImmunotherapyProductType:RecombinantProteinDatashownislot-specific.Contactusforspecificinformationonotherlots.

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