首页 > BPS Bioscience > Bpsbioscience/BAZ2B Inhibitor Screening Assay Kit/32600/384 reactions

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Bpsbioscience/BAZ2B Inhibitor Screening Assay Kit/32600/384 reactions

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32600

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4000-520-616

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Description:TheBAZ2BInhibitorScreeningAssayKitisdesignedtomeasuretheinhibitionofBAZ2Bbindingtoitssubstrate.ThekeytotheBAZ2BInhibitorScreeningAssayKitisthehighlyspecificbindingoftheBAZ2Bbromodomaintotheacetylatedhistonesubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingBAZ2Bandaninhibitorofchoiceisincubatedwiththebiotinylatedsubstrateforthirtyminutes.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.SuppliedAs:KitcomesinaconvenientAlphaLISA®format,withbiotinylatedhistonepeptidesubstrate,assaybuffer,detectionbufferandpurifiedHis-BAZ2Bbromodomaintoperformatotalof384enzymereactions.Contraindications:DMSOabove0.5%.OnlylimitedamountsofDMSOcanbeincluded,asithasbeenshowntodisruptBRD-ligandinteraction.AvoidgreenandbluedyesthatabsorblightintheAlphascreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphascreen®assays.Format:COMPONENTS:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:Atleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):Usefulforthestudyofbromodomainbindingassays,screeningbromodomaininhibitorsandselectivityprofiling.Reference(s):Jones,M.H.,etal.,Genomics2000;63(1):40.Notes:MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:NickelChelateAlphaLISA®AcceptorBeads,5mg/ml(Perkinelmer#AL108C)AlphaScreen®Streptavidin-conjugateddonorbeads,5mg/ml(PerkinElmer#6760002)Optiplate-384(PerkinElmer#6007290)AlphaScreen®microplatereaderAdjustablemicropipettorandsteriletipsWarning(s):Avoidfreeze/thawcycles.ScientificCategory:BromodomainProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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