首页 > BPS Bioscience > Bpsbioscience/ARE Reporter Kit (Nrf2 Antioxidant Pathway )/60514/500 reactions

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Bpsbioscience/ARE Reporter Kit (Nrf2 Antioxidant Pathway )/60514/500 reactions

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60514

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Background:TheNrf2antioxidantresponsepathwayplaysanimportantroleinthecellularantioxidantdefense.Nrf2,abasicleucinezippertranscriptionfactor,inducestheexpressionofantioxidantandphaseIIenzymesbybindingtotheARE(antioxidantresponseelement)regionofthegenepromoter.Underbasalconditions,Nrf2isretainedinthecytosolbybindingtothecytoskeletalproteinKeap1.UponexposuretooxidativestressandotherAREactivators,Nrf2isreleasedfromKeap1andtranslocatestonucleus,whereitcanbindtotheARE,leADIngtotheexpressionofantioxidantandphaseIIenzymesthatprotectthecellfromoxidativedamage.Description:TheAREReporterkitisdesignedformonitoringtheactivityoftheNrf2antioxidantpathwayinculturedcells.Thekitcontainsatransfection-readyAREluciferasereportervector,whichisanNrf2pathway-responsivereporter.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedAREresponsiveelementslocatedupstreamofaminimalpromoter.TheAREreporterispremixedwithaconstitutivelyexpressingRenilla(seapansy)luciferasevectorthatservesasaninternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwithconstitutivelyexpressingRenillaluciferasevectorasnegativecontrol.Thenon-inducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thisnegativecontroliscriticaltodeterminingpathway-specificeffectsandbackgroundluciferaseactivity.UniProtQ16236Synonym(s):AREreporter,Nrf2reporter,NrfSuppliedAs:Reporter:AREluciferasereportervector+constitutively-expressingRenillaluciferasevectorNegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevectorConcentration:BoththeReporterandNegativeControlReporterareprovidedat60ngDNA/µl(500µleach)Format:assaykitInstructionsforuse:Seedatasheetinsertfordetailedtransfectionandassayprotocols.Storage/StABIlity:Stableatleast12monthsfromdateofreceipt,whenstoredasdirected(-20°C)Application(s):•MonitorNrf2antioxidantresponsepathwayactivity.•ScreenforactivatorsorinhibitorsoftheNrf2antioxidantresponsepathway.•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheNrf2pathway.Reference(s):ApplicationReference(s):1. Mecanismosneuroquímicosassociadosaosefeitostipo-antidepressivoeneuroprotetordeinosina(2017)2. Protectiveeffectsofrosmarinicacidagainsthydrogenperoxide‑inducedcellularsenescenceandtheinflammatoryresponseinnormalhumandermalfibroblasts(2017)3. Pharmacologicalstimulationofnuclearfactor(erythroid-derived2)-like2translationactivatesantioxidantresponses(2017)3. RoleofextracellularHydrogenperoxideinregulationofironhomeostasisgenesinneuronalcells:Implicationinironaccumulation(2015)Notes:Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.Warning(s):Avoidfreeze/thawcyclesProductType:ReporterAssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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