首页 > BPS Bioscience > Bpsbioscience/CSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway)/79379/500 reactions

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Bpsbioscience/CSF1R / SRE Reporter Kit (MAPK/ERK Signaling Pathway)/79379/500 reactions

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

79379

品牌：

BPS Bioscience

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

Background:ColonyStimulatingFactor1Receptor(CSF1R,CD115,M-CSF-R)isthereceptorforthecytokineCSF1(MCSF;CSF-1),whichcontrolsthegrowth,function,anddifferentiationofmacrophages.ThisinteractiondrivesthedevelopmentandsurvivalofTumor-AssociatedMacrophages(TAMs),whichinturnsuppressthelocalimmuneresponsetothecancer.AnalternativeligandisIL-34,whichhasbeenimplicatedinneuroprotectiveandsurvivalsignalsfollowingbraininjuryandneurodegeneration.Asatyrosinekinasetransmembranereceptor,activationofCSF1Rbyitsligandinitiatesavastarrayofintracellularactivity,includingactivationoftheMAPK/ERKsignalingpathway.WhenphosphorylatedbyERK,Elk1formsacomplexwithSerumResponseFactor(SRF)andbindstoSerumResponseElement(SRE),resultingintheexpressionofnumerousmitogen-inducIBLegenes.Description:TheCSF1RSREReporterKitisdesignedformonitoringtheactivityoftheCSF1Rsignalingpathwayinculturedcells.Thekitcontainsatransfection-readyvectorforCSF1RandSREluciferasereportervector.Uponligandbinding,activeCSF1RwillinitiatetheMAPK/ERKsignalingpathway,leADIngtoexpressionoftheSRE-controlledreporter.ThisreportercontainsthefireflyluciferasegeneunderthecontrolofmultimerizedSREresponsiveelementslocatedupstreamofaminimalpromoter.TheSREreporterispremixedwithaconstitutively-expressingRenillaluciferasevectorthatservesasaninternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-induciblefireflyluciferasevectorpremixedwithconstitutively-expressingRenillaluciferasevectorasanegativecontrol.Thenon-inducibleluciferasevectorcontainsthefireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticalfordeterminingpathway-specificeffectsandthebackgroundluciferaseactivity.MaterialsRequiredButNotProvided:•Mammaliancelllineandappropriatecellculturemedium•96-welltissuecultureplateor96-welltissueculture-treatedwhiteclear-bottomassayplate•Transfectionreagentformammaliancellline[WeuseLipofectamine™2000(Invitrogen#11668027)+Opti-MEMIReducedSerumMedium(Invitrogen#31985-062).However,othertransfectionreagentsworkequallywell.]•RecombinantHumanCSF1(BPSBioscience,#90215-B)orRecombinantHumanIL34(Biolegend,#577904)•Lowserum(0.5%FBS)growthmediumoptimizedforparentalcelltypeForHEK293celllinesuseBPSMedium8(BPSBioscience,#79385):MEMmedium(Hyclone#SH30024.01)supplementedwith0.5%FBS(Invitrogen#26140-079),1%non-essentialaminoacids(Hyclone#SH30238.01),1mMNapyruvate(Hyclone#SH30239.01),1%Penicillin/Streptomycin(HycloneSV30010.01)•DualLuciferaseAssaySystem:DualLuciferase(Firefly-Renilla)AssaySystem(BPSBioscience,#60683):Thissystemassayscellsdirectlyingrowthmedium.Itcanbeusedwithanyluminometer.Automatedinjectorsarenotrequired.•LuminometerSynonym(s):FMS,CD115SuppliedAs:Eachkitcontainssufficientreagentsfor500assaysusinga96-wellplate.Format:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:Storecelllineinliquidnitrogenimmediatelyuponreceipt.Storeothercomponentsasdescribedbelow.Stableforatleast6monthsfromdateofreceiptwhenstoredasdirected.Application(s):•MonitorCSF1R/MAPK/ERKsignalingpathwayactivityandSRF-mediatedactivity.•ScreenforactivatorsorinhibitorsoftheCSF1R/MAPK/ERKsignalingpathway.•StudyeffectsofRNAiorgeneoverexpression.Reference(s):1.Cannarile,MichaelA.,etal.“Colony-StimulatingFactor1Receptor(CSF1R)InhibitorsinCancerTherapy.”J.Immunother.Cancer,5(1):53(2017)2.Liu,Yang,andXuetaoCao.“TheOriginandFunctionofTumor-AssociatedMacrophages.”CellularMolec.Immunology,12(1):1–4(2014).3.Luo,Jian,etal.“Colony-StimulatingFactor1Receptor(CSF1R)SignalinginInjuredNeuronsFacilitatesProtectionandSurvival.”J.Exp.Med.,210(1):157–172(2013).4.Yao,G.-Q.,etal.“CSF-1InducesFosGeneTranscriptionandActivatestheTranscriptionFactorElk-1inMatureOsteoclasts.”Calc.TissueInt.,76(5):371–378(2005).Warning(s):Avoidfreeze/thawcycles.ScientificCategory:ImmunotherapyProductType:Cell-BasedAssaysDatashownislot-specific.Contactusforspecificinformationonotherlots.

bpsbioscience
简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。