首页 > BPS Bioscience > Bpsbioscience/ONE-Step™ Luciferase Assay System - 100 ml/60690-2/100 ml

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Bpsbioscience/ONE-Step™ Luciferase Assay System - 100 ml/60690-2/100 ml

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60690-2

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BPS Bioscience

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4000-520-616

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Background:Luciferaseisthegeneraltermgiventoaclassofoxidativeenzymesthatcatalyzereactionsthatgiveofflight,aprocessknownasbioluminescence.InBIOLOGy,researcherscantakeadvantageofthisreactionanduseitasareadoutforvariousbiologicalprocesses.Thishasperhapsbeenexploitedmostinluciferasereportercelllineswhereapromoterregionfromageneofinterestisplacedimmediatelyupstreamofthecodingsequenceforluciferase.Inthissystem,transcriptionalactivationofthegeneofinterestleadstoalevelofluciferaseexpressionthatisproportionaltothelevelofgeneactivation.Thelevelofactivationisthenassessedbylysingthecellsandaddingtheluciferasesubstrate,D-luciferin.UsingtheOne-StepLuciferaseAssaySystemfromBPSBioscience,thisisaccomplishedinasinglestepasthelysisbufferhasbeenformulatedwithD-luciferin.Afterabriefincubationperiod,bioluminescenceisreadonaluminometer.Description:TheOne-StepLuciferaseAssaySystemisdesignedtobeusedforhigh-throughput,sensitivequantitationoffireflyluciferaseactivityinmammaliancellculture.Thereagentconsistsoftwocomponents,aLuciferaseReagentBuffer(ComponentA)andLuciferaseReagentSubstrate(ComponentB).ComponentAandComponentBarecombinedtoformaworkingsolutionthatcontainsallthenecessarycomponentsforcelllysisandluciferasequantitation.Thisassaysystemhasseveralfeatures:•Sensitive–highlysensitivedetectionoffireflyluciferaseactivity.•Stable–thesignaloutputisstableformorethantwohours,providingflexibilitywithregardtoincubationtime•Convenient–simpleone-step,homogeneousprotocol.•High-throughput–one-stephomogeneousprotocolminimizeshandlingstepstosupporthigh-throughputscreeningapplications•Compatibility–workswellwithavarietyofcommonmediacontaining0-10%serumandphenolred.•Instrumentation–doesnotrequirealuminometerwithinjectors.Synonym(s):Luciferin,Fireflyluciferase,luciferasebufferFormat:COMPONENTS:Instructionsforuse:Seeproductdatasheetfordetailedprotocol.Storage/StABIlity:Atleast6monthswhenstoredasdirected.Uponfirstthaw,storeinaliquotsat-20°C.Thereagentmaybesubjectedtoseveralfreeze/thawcycleswithnoeffectonfunctionality,butitisrecommendedthatfreeze/thawcyclesbeavoidedwheneverpossIBLe.Application(s):•Monitorfirefly(Photinuspyralis)luciferaseactivityinculturedmammaliancells.•High-throughputscreeningusingluciferasereportercelllinesNotes:•Thereagenthasbeenvalidatedina96-wellformat.Otherformatswillrequirescalingandoptimizationbytheend-user.•LuciferaseReagentBuffermustbeat~roomtemperature(20-25°C)beforeuse.•LuciferaseAssayReagentshouldbeaddedtocellsforatleast5minutesbeforemeasuringluminescencetoallowcompletecelllysis.•Formaximallightintensity,measuresampleswithin1hourofreagentaddition.•Avoidexposingtoexcessiveheatorlightduringincubation.•Differentcelllinesmayexhibitvariationinlysisabilityand/orluminescencesignalandmayrequireslightoptimizationbytheend-user.•Luminescencesignalisaffectedbyassayconditions.Resultsshouldbecomparedbetweensamplesmeasuredusingthesamecelltypeandmedia/serumcombinations.•Toanalyzemultipleplates,includeacommoncontrolsampleineachplateandnormalizetheluminescenceofeachplatetothecontrolcontainedinthesameplate.•Backgroundluminescenceisacharacteristicofluminometerperformance,therefore,backgroundluminescencemustbesubtractedfromallreADIngsforaccuracy. Warning(s):Protectfromlight.Avoidfreeze/thawcycles.ScientificCategory:Cell-BasedAssayKitsProductType:ReagentDatashownislot-specific.Contactusforspecificinformationonotherlots.

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简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。