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FabGennix/ACTH Antibody/ACTH-101AP
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DescriptionQA_1Q&ARatings (1)p { margin:0pt 0pt 10pt }table { margin-top:0pt; margin-bottom:10pt }.BalloonText { margin-bottom:0pt; line-height:normal; font-family:Tahoma; font-size:8pt }.ListParagraph { margin-left:36pt; margin-bottom:10pt; line-height:115%; font-size:11pt }span.BalloonTextChar { font-family:Tahoma; font-size:8pt }span.Hyperlink { text-decoration:underline; color:#0000ff }.TableGrid { margin-bottom:0pt; line-height:normal }General InformationProductACTH AntibodyDescriptionAffinity Purified Adrenocorticotropin Hormone Antibody Verified ApplicationsELISA, IHC-Fr, IHC-P, IP, WBHostRabbitSpecies Cross ReactivityHuman, Monkey,Mouse, RatPredicted: Chicken, Chimpanzee, Cow, Dog, Goat, Guinea pig, Macaque Monkey, Orangutan, SheepImmunogenSynthetic peptide corresponding to ACTH aa 138-176.Sequence:SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFAccession #NCBI: AAX36901Physical PropertiesQuantity100 µgVolume200 µlConcentration0.67-0.68 µg/µl in antibody stabilization bufferFormAffinity PurifiedClonalityPolyclonalStorage-20⁰C for long term storageApplication ProtocolConfocal Microscopy1:250Immunocytochemistry1:250Immunofluorescence1:200Immunohistochemistry1:250Immunoprecipitation1:200Western Blot1:1000 PredictedMolecular weight: 29 kDaProteinUniprot #UPI00004EBE53OverviewStimulates adrenal glands and release cortisol.Molecular FunctionGPCR; Hormone activitySubcellular LocationSecretedExpressionACTH and MSH are produced by the pituitary glandStructureBelongs to POMC familyAlternative NomenclatureAlpha Melanocyte Stimulating Hormone antibodyBeta Endorphin antibodyBeta Lipotropin antibodyBeta Melanocyte Stimulating Hormone antibodyBeta-endorphin antibodyCLIP antibodyCorticotropin Like Intermediary Peptide antibodyMet-enkephalin antibodyMSH antibodyNPP antibodyOpiomelanocortin prepropeptide antibodyPOC antibodyPOMC antibodyTetracosactide antibodyProtein information supplied by UniProtp { margin:0pt }li { margin-top:0pt; margin-bottom:0pt }.ListParagraph { margin-left:36pt; margin-bottom:0pt; line-height:normal; font-family:Calibri; font-size:11pt }.NormalWeb { margin-top:5pt; margin-bottom:5pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }span.Hyperlink { text-decoration:underline; color:#004a91 }Your datasheets states this antibody was raised against "aa 1-70 of ACTH." However, ACTH (AKA corticotropin) is only a 38 aa protein (aa 138-176 of POMC) that is formed from cleavage of POMC, see the uniprot file below. I wanted to confirm if this antibody was raised against amino acids 1-70 or 138-176 of POMC.http://www.uniprot.org/uniprot/P01189 The ACTH antibodies were raised against 39 amino acid ACTH hormone. The Specification sheet specifies the rangefrom where the peptide was selected. In this case the 39 AA are the complete ACTH hormone. ACTH human1-39SYS MEH FRW GKP VGK KRR PVK VYP NGA EDE SAE AFP LEFThe peptide was conjugated with a carrier protein for making ACTH antibodies.p { margin:0pt }li { margin-top:0pt; margin-bottom:0pt }.ListParagraph { margin-left:36pt; margin-bottom:0pt; line-height:normal; font-family:Calibri; font-size:11pt }.NormalWeb { margin-top:5pt; margin-bottom:5pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }span.Hyperlink { text-decoration:underline; color:#004a91 }Your datasheets states this antibody was raised against "aa 1-70 of ACTH." However, ACTH (AKA corticotropin) is only a 38 aa protein (aa 138-176 of POMC) that is formed from cleavage of POMC, see the uniprot file below. I wanted to confirm if this antibody was raised against amino acids 1-70 or 138-176 of POMC.http://www.uniprot.org/uniprot/P01189 The ACTH antibodies were raised against 39 amino acid ACTH hormone. The Specification sheet specifies the rangefrom where the peptide was selected. In this case the 39 AA are the complete ACTH hormone. ACTH human1-39SYS MEH FRW GKP VGK KRR PVK VYP NGA EDE SAE AFP LEFThe peptide was conjugated with a carrier protein for making ACTH antibodies. Customer ratings for ACTH Antibody Number of ratings: 1Average rating: 5Excellentfrom Anonymous on 11/13/2015Western BlotPig Brain purified proteinReduced Denaturing (4-12% Bis-Tris)10 ug sample loadedBSA used as blocking agent for 1 hour. 3% at 22C.1:500 primary antibody dilution for 1 hour at 22C.DCIP/NBT detection methodBands: Specific: 5, 10, 15, 20 kDa.

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