DescriptionPublicationsSpecification SheetQA_1Q&ARatings (1)p { margin:0pt 0pt 10pt }table { margin-top:0pt; margin-bottom:10pt }.BalloonText { margin-bottom:0pt; line-height:normal; font-family:Tahoma; font-size:8pt }span.BalloonTextChar { font-family:Tahoma; font-size:8pt }span.Hyperlink { text-decoration:underline; color:#0000ff }.TableGrid { margin-bottom:0pt; line-height:normal }General InformationProductPhospho-MERTK AntibodyDescriptionAffinity Purified Phosphorylated Tyrosine-protein kinase Mer AntibodyVerified ApplicationsCM, ELISA, FC, ICC, IF, IHC, WBHostRabbitSpecies Cross ReactivityHuman, Monkey, Mouse, RatImmunogenSynthetic peptide corresponding to amino acids 746-757 ofHuman MERTK.Sequence:CKKIYpSGDYpYpRQGAccession #NCBI: NP_075232.1Physical PropertiesQuantity100 µgVolume200 µlConcentration0.63 µg/µl in antibody stabilization bufferFormAffinity PurifiedClonalityPolyclonalStorage-20⁰C for long term storageApplication ProtocolConfocal Microscopy1:250DB1:10,000ELISA1:10,000Immunocytochemistry1:250Immunofluorescence1:250Immunohistochemistry1:250Western Blot1:750. Observed band of 180kDaProteinUniprot #P57097OverviewReceptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules.Molecular FunctionKinase, Receptor, Transferase, Tyrosine-protein kinaseSubcellular LocationMembrane; Single-pass type I membrane proteinStructureInteracts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner.Alternative Nomenclaturec MER antibodyc mer proto oncogene tyrosine kinaseEykMERMERPENMertkMERTK c-mer proto-oncogene tyrosine kinasenmf12NykProto oncogene tyrosine protein kinase MERReceptor tyrosine kinase MerTKRP38STK kinaseTyrosine-protein kinase MerProtein information supplied by UniProtp { margin:0pt 0pt 10pt }li, table { margin-top:0pt; margin-bottom:10pt }.ListParagraph { margin-left:36pt; margin-bottom:10pt; line-height:115%; font-size:11pt }span.Hyperlink { text-decoration:underline; color:#0000ff }.TableGrid { margin-bottom:0pt; line-height:normal }FabGennix Phospho-MerTK Antibody has been used in 26Publications Applications: Confocal microscopy, Flow cytometry, Immunocytochemistry, Immunohistochemistry Immunofluorescence, and Western blotReactivity: Human, Mouse, and RatZCai, Bishuang, et al. "MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity." Sci. Signal. 11.549 (2018).PMID:30254055Applications:Western BlotReactivity: HumanZhang, Bing, et al. "Mer receptor tyrosine kinase negatively regulates lipoteichoic acid-induced inflammatory response via PI3K/Akt and SOCS3." Molecular immunology 76 (2016).PMID: 27419619Applications: Western BlotReactivity: MouseDas, Amitava, et al. "Correction of MFG-E8 resolves inflammation and promotes cutaneous wound healing in diabetes." The Journal of Immunology 196.12 (2016).PMID: 27194784Applications: Western BlotReactivity: MouseCai, Bishuang, et al. "MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation." Proceedings of the National Academy of Sciences 113.23 (2016).PMID: 27199481Applications: Western BlotReactivity: MouseGinisty, Aurélie, et al. "Evidence for a Subventricular Zone Neural Stem Cell Phagocytic Activity Stimulated by the Vitamin K‐Dependent Factor Protein S." STEM CELLS 33.2 (2015).PMID: 25308179Applications: Western BlotReactivity: RatNguyen, Khanh-Quynh N., et al. "Overexpression of MERTK receptor tyrosine kinase in epithelial cancer cells drives efferocytosis in a gain-of-function capacity." Journal of Biological Chemistry 289.37 (2014).PMID: 25074939Applications: Western BlotReactivity: HumanLaw, Ah-Lai, et al. "Cleavage of Mer Tyrosine Kinase (MerTK) from the Cell Surface Contributes to the Regulation of Retinal Phagocytosis." Journal of Biological Chemistry (2014).PMID: 25538233Applications: Western BlotReactivity: MouseSuleiman, Lutfi, Claude Négrier, and Habib Boukerche. "Protein S: A multifunctional anticoagulant vitamin K-dependent protein at the crossroads of coagulation, inflammation, angiogenesis, and cancer."Critical Reviews in oncology/hematology88.3 (2013).PMID: 23065156Applications: Western BlotReactivity: MouseWang, Y, et al. "Mer Receptor Tyrosine Kinase Promotes Invasion and Survival In Glioblastoma Multiforme."Oncogene32.7 (2013): 872-882.PMID: 22469987Applications: Confocal Microscopy, Immunofluorescence, Immunohistochemistry, Western BlotReactivity: HumanChoi, Ji-Yeon, et al. "Upregulation of Mer receptor tyrosine kinase signaling attenuated lipopolysaccharide-induced lung inflammation." Journal of Pharmacology and Experimental Therapeutics 344.2 (2013).PMID: 23197771Applications: Western BlotReactivity: MouseYefimova, Marina G. et al. “A Chimerical Phagocytosis Model Reveals the Recruitment by Sertoli Cells of Autophagy for the Degradation of Ingested Illegitimate Substrates.” Autophagy9.5 (2013).PMCID: 3669177Applications: Confocal Microscopy, Immunocytochemistry, Immunofluorescence, Western BlotReactivity: HumanClick Here for the complete list of all FabGennix Phospho-MerTK Antibody publicationsPublishing research using PMKT-140AP? Please let us know, we can cite the reference in this datasheet.To view the Specification Sheet for Phospho-MerTKAntibody, please click the link below:Phospho-MerTK Antibody| FabGennixp { margin:0pt 0pt 8pt }li { margin-top:0pt; margin-bottom:8pt }.ListParagraph { margin-left:36pt; margin-bottom:0pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }.NormalWeb { margin-top:5pt; margin-bottom:5pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }span.Hyperlink { text-decoration:underline; color:#0000ff }I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).I incubated the blot with the above mentioned antibody for overnight at 4 C on rotating shaker diluted 1:750 in BSA buffer (5% BSA in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 ).Next day, followed by 6 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20. As a secondary antibody I used a anti-rabbit HRP conjugate from cell signaling, diluted 1:10000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 6 times as above and developed using ECL.After phosphoMer detection, the blot was washed 6 times with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 was analysed for total MERTK using the AF891 from R & D system. Similar process was used for total MERTK also.Results-When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.We know now for sure that:phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagentsI am not sure about the characteristics of non-phospho-MER antibodiesused in these experiments. Do they recognize all forms of differentially modified MER or just recognize the core proteins and does not recognize the fully mature MER proteins in the samples?Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.In some samples our Phospho-mer antibody (PMKT-140AP) also recognize a smaller (just above 90kDa) band. The MW of these proteins are apparent in relation to the MW standards and cannot be considered as actual size of the proteins. These numbers will vary quite significantly on different pore size gels, and sometimes even when different companies MW markers are used. We also know that calculated MW will also vary when colored and non-color MW makers are compared on the same gel. p { margin:0pt 0pt 8pt }li { margin-top:0pt; margin-bottom:8pt }.ListParagraph { margin-left:36pt; margin-bottom:0pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }.NormalWeb { margin-top:5pt; margin-bottom:5pt; line-height:normal; font-family:"Times New Roman"; font-size:12pt }span.Hyperlink { text-decoration:underline; color:#0000ff }I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).I incubated the blot with the above mentioned antibody for overnight at 4 C on rotating shaker diluted 1:750 in BSA buffer (5% BSA in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 ).Next day, followed by 6 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20. As a secondary antibody I used a anti-rabbit HRP conjugate from cell signaling, diluted 1:10000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 6 times as above and developed using ECL.After phosphoMer detection, the blot was washed 6 times with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 was analysed for total MERTK using the AF891 from R & D system. Similar process was used for total MERTK also.Results-When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.We know now for sure that:phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagentsI am not sure about the characteristics of non-phospho-MER antibodiesused in these experiments. Do they recognize all forms of differentially modified MER or just recognize the core proteins and does not recognize the fully mature MER proteins in the samples?Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.In some samples our Phospho-mer antibody (PMKT-140AP) also recognize a smaller (just above 90kDa) band. The MW of these proteins are apparent in relation to the MW standards and cannot be considered as actual size of the proteins. These numbers will vary quite significantly on different pore size gels, and sometimes even when different companies MW markers are used. We also know that calculated MW will also vary when colored and non-color MW makers are compared on the same gel. Customer ratings for Phospho-MERTK Antibody Number of ratings: 1Average rating: 55 startsfrom Anonymous on 1/28/2017I have been working with this antibody for the last 3 years. It works very well.AccessoriesProductNoteStatusPricePhospho-MERTK Antibody FITCPMKT-FITC$395.00*Phospho-MERTK Antibody BIOTINPMKT-BIOTIN$395.00*Phospho-MERTK Blocking PeptideP-PMKT$180.00*Phospho-MERTK Positive ControlPC-PMKT$205.00** Shipping OptionsBulk and Special Orders QuoteDisplay accessory detailsWe also recommendMKT-121APMERTK Antibody $295.00* MKT-101APMERTK Antibody $295.00* MKT-112APMERTK Antibody $295.00* TYRO3-101APTYRO3 Antibody $295.00* * Shipping OptionsBulk and Special Orders QuoteCustomers who bought this product also boughtMERTK Antibody$295.00* Phospho-MERTK Positive Control$205.00* * Shipping OptionsBulk and Special Orders QuoteBrowse these categories as well:Neurotransmission, Tyrosine Kinases, Vision, Primaries, Receptor Tyrosine Kinases

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