产品说明
FormatLyophilizedQuantity120 µlReconstitutionFor reconstitution add 100 µl of milliQ water. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.Tested applicationsWestern blot (WB)Recommended dilutionStandard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.Expected | apparent MW27 kDa
Agrisera针对植物蛋白质的保守氨基酸序列,Agirsera还**开发了数十种通用抗体(global antibodies),这类抗体几乎可以与所有的高等植物及藻类产生免疫反应,可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体,Rubisco即核酮糖二磷酸羧化酶,是植物组织中广泛存在的一种高丰度蛋白,在一些植物学WB检测时,经常被用作内参抗体使用。
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