首页 > Agrisera > Agrisera/Donkey anti-Goat IgG (H&L), DyLight® 350 conjugated/AS12 2492/

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Agrisera/Donkey anti-Goat IgG (H&L), DyLight® 350 conjugated/AS12 2492/

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AS122492

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productinformationBackgroundDonkeyanti-GoatIgG(H&L),DyLight®350ConjugatedisasecondaryantibodyconjugatedtoDyLight®350,whichbindstoGoatIgG(H&L)inimmunologicalassays.DyLight®350hasAmax=353nm,Emax=432nm.AntibodiesarepurifiedusingsolidphaseGoatIgG(H&L)DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries.ImmunogenPurifiedGoatIgG,wholemoleculeHostDonkeyClonalityPolyclonalClonePurityAffinitypurifieddonkeyIgGFormatLyophilizedQuantity1mgReconstitutionForreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.StorageStorelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration.Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity.Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.TestedapplicationsRelatedproductsotherDyLight®anti-GoatsecondaryantibodiesAdditionalinformationConjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative.Basedonimmunoelectrophoresis,thisantibodyreactswith:,heavychainsongoatIgG,lightchainsonallgoatimmunoglobulinsNoreactivityisobservedto,non-immunoglobulingoatserumproteins.BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.applicationinformationRecommendeddilution1:20-1:2,000formostapplicationsExpected|apparentMWConfirmedreactivityPredictedreactivityNotreactiveinAdditionalinformationSelectedreferencestobeaddedwhenavailable

Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。