首页 > Agrisera > Agrisera/Goat anti-Mouse IgG (H&L), F(ab)2 fragment, DyLight® 800 conjugated, min. cross-reactivity to bovine, goat, hu

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Agrisera/Goat anti-Mouse IgG (H&L), F(ab)2 fragment, DyLight® 800 conjugated, min. cross-reactivity to bovine, goat, hu

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productinformationBackgroundGoatanti-MouseIgG(H&L)-F(ab)"2fragment,DyLight®800Conjugated,min.cross-reactivitytobovine,goat,human,rabbit,orratIgGisasecondaryantibodyconjugatedtoDyLight®800,whichbindstoMouseIgG(H&L)-F(ab)"2fragmentinimmunologicalassays.DyLight®800hasAmax=777nm,Emax=794nm.AntibodiesarepurifiedusingsolidphaseMouseIgG(H&L)DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries.ImmunogenPurifiedMouseIgG,wholemoleculeHostGoatClonalityPolyclonalClonePurityAffinitypurifiedgoatIgGFormatLyophilizedQuantity0.5mgReconstitutionForreconstitutionpleaseadd0.55mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.StorageStorelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration.Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity.Forexample,ifyouhavereconstituted0.5mgofantibodyin0.55mlofsterilewateradd0.55mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.TestedapplicationsRelatedproductsotherDyLight®anti-MousesecondaryantibodiesAdditionalinformationConjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative.Basedonimmunoelectrophoresis,thisantibodyreactswith:heavy(γ)chainsonmouseIgG,lightchainsonallmouseimmunoglobulinsNoreactivityisobservedto:non-immunoglobulinmouseserumproteins,IgGfrombovine,goat,human,rabbitorratapplicationinformationRecommendeddilution1:20-1:2,000formostapplicationsExpected|apparentMWConfirmedreactivityPredictedreactivityNotreactiveinAdditionalinformationSelectedreferencestobeaddedwhenavailable

Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。