首页 > Agrisera > Agrisera/Cat | Catalase (peroxisomal marker) (100 µl)/AS09 501-100/

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Agrisera/Cat | Catalase (peroxisomal marker) (100 µl)/AS09 501-100/

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AS09501-100

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Agrisera

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productinformationBackgroundCatalaseisanenzymefoundinmostlivingorganismswhichiscatalazyingdecompositionofhydrogenperoxidetowaterandoxygen.Inplantcellscatalaseisfoundinperoxisomes.Thisenzymeisinvolvedinphotorespirationandsymbioticnitrogenfixation.ImmunogenKLH-conjugatedpeptidechosenfromknowplantcatalasesequencesincludingArABIdopsisthalianaisoforms:catalase-1(Q96528,At1g20630),catalase-2(P25819,At4g35090),catalase-3(Q42547,At1g20620);HostRabbitClonalityPolyclonalClonePuritySerumFormatLyophilizedQuantity2x50µlReconstitutionStoragestoreat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.Testedapplicationswesternblot(WB)RelatedproductsAS08374|anti-KatG|catalaseperoxidase(HPI),cyanobacterialPlantproteinextractionbufferSecondaryantibodiesAdditionalinformationThisantibodyisrecognizingallthreeisoformsofArabidopsisthalianacatalase.applicationinformationRecommendeddilution1:1000withstandardECL(WB)Expected|apparentMW57|55kDaConfirmedreactivityArabidopsisthaliana,Brassicaoleracea,Hordeumvulgare,Nicotianabentamina,Nicotianatabacum,Oryzasativa,Solanumlycopersicum,Spinaciaoleracea,Zeamays,VitisviniferaPredictedreactivitydicotsincluding:Cucumissativus, Gossypiummexicanum,Glycinemax,trees:PopulusjackiiNotreactiveinChlamydomonasreinhardtiiAdditionalinformationToobtainreactivitywithSolanumlycopersicumureagelneedstobeapply.Please,contactusformoredetails.SelectedreferencesKatayaetal.(2015).ProteinPhosphatase2AHoloenzymeIsTargetedtoPeroxisomesbyPiggybackingandPositivelyAffectsPeroxisomalβ-Oxidation.PlantPhysiol.2015Feb;167(2):493-506.doi:10.1104/pp.114.254409.Pilatietal.(2014).TheonsetofgrapevineberryripeningischaracterizedbyROSaccumulationandlipoxygenase-mediatedmembraneperoxidationintheskin.BMCPlantBiol.2014Apr2;14(1):87.Blumeetal.(2013).ApossIBLeroleforthechloroplastpyruvatedehydrogenasecomplexinplantglycolateandglyoxylatemetabolism.PhytochemistryAug2.Lietal.(2013).LESIONSIMULATINGDISEASE1InteractswithCatalasestoRegulateHypersensitiveCellDeathinArabidopsis.PlantPhysiol.Aug19.Maroketal.(2013).Adrought-sensitivebarleyvarietydisplaysoxidativestressandstronglyincreasedcontentsinlow-molecularweightantioxidantcompoundsduringwaterdeficitcomparedtoatolerantvariety.J.PlantPhysioology,Feb8.10µgoftotalproteinfromArabidopsisthalianaCol0(1),Cat2-(Col0)(2),Ler0(3),Cat2-(Ler0)(4),Zeamays(5),Oryzasativa(6),Brassicaoleracea(7),Nicotianabentamina(8)wereextractedwith60mMTrispH6.9,10mMDTT,20%glycerol,1mmPMSFwereseparatedon12.5%SDS-PAGEandblotted1htoPVDF.Blotwasblockedwith3%skimmilkinPBS+0.05%Tween20for1hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:1000for1hatRTwithagitationinthesamebuffer.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflythreetimes,thenwashed3timesfor5mininPBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorserADIshperoxidaseconjugated,Agrisera,AS09602)dilutedto1:50000in3%skimmilkinPBS+0.05%Tween20for1hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor1minwithWesternLightningPlus-ECL(Perkinelmer)accordingtothemanufacturersinstructions.Exposuretimewas 5min.inChemiDocXRS+(Biorad).CourtesyofBrigittevandeCotte,GentUniversity,Belgium

Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。