首页 > Agrisera > Agrisera/H+ATPase | Plasma membrane H+ATPase (100 µl)/AS07 260-100/

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Agrisera/H+ATPase | Plasma membrane H+ATPase (100 µl)/AS07 260-100/

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productinformationBackgroundThePlasmaMembraneH+ATPaseisafamilyofproteinsofca.100kDathatarebelievedtobeexclusivetotheplasmamembranesofplantsandfungi.TheproteinisanchoredwithinBIOLOGicalmembranewhichcreatesanelectRochemicalgrADIentusedasanenergysourceandisessentialforuptakeofmostmetabolitesandplantresponsestoenvironment,forexamplemovementofleaves.ImmunogenKLH-conjugatedsyntheticpeptideexposedtocytoplasminH+ATPasemodel,derivedfromavailablediandmonocot,fern,mossesandalgalplasmamembraneATPasesequencesincludingArABIdopsisthalianaATPase1(UniProt:P20649,TAIR:At2g18960)andATPase2(UniProt: P19456,TAIR:At4g30190),3(UniProt:P20431,TAIR:At5g57350),4(UniProt: Q9SU58,TAIR:At3g47950),6(UniProt: Q9SH76,TAIR:At2g07560),7(UniProt: Q9LY32,TAIR:At3g60330),8(UniProt: Q9M2A0,TAIR:At3g42640),9(UniProt: Q42556,TAIR:At1g80660),11(UniProt:Q9LV11,TAIR:At5g62670)ofArabidopsisthalianaandhydrogenATPaseofChlamydomonasreinhardtii(Q9FNS3)HostRabbitClonalityPolyclonalClonePuritySerumFormatLyophilizedQuantity2x50µlReconstitutionForreconstitutionadd50µlofsterilewatertoeachtubeStoragestorelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.Donotstorethisantibodyin4°C.Testedapplicationswesternblot(WB),immunofluorescence(IF),immunolocalization(IL)RelatedproductsAS07213|anti-V-ATPaserabbitantibodyAS07260PRE|H+ATPase|plasmamembraneH+ATPase,pre-immuneserumAS132671|H+ATPaseplasmamembraneH+ATPase(chickenantibody)antibodiestomembranetransportsystemrecommendedsecondaryantibodyAdditionalinformationcellular[compartmentMarker]forplasmamembraneapplicationinformationRecommendeddilution1:1000-1:5000withstandardECLOR1:10000withECL-Advance,enhancedchemiluminescence(WB),1:600-1:1000(IF),1:100(IL)Expected|apparentMW95kDa(Arabidopsisthaliana)Confirmedreactivitydicotsincluding:Arabidopsisthaliana,Cucumissativus,Cucurbitamoschata,Glycinemax(weak),Kandeliaobovata,Loliumperenne,Lycopersiconesculentum,Marchantiapolymorpha,Medicagotruntata,Nicotianatabacum,Noccaeacaerulescens,PhalenopsisSogoYukidiancultivarV3,Ricinuscommunis,Spinaciaoleracea,monocotsinlcuding:Hordeumvulgare,Oryzasativa,Zeamays,trees:Piceaabies,Populustremula,Pterisvittata(fern),algae:ChlamydmonasreinhardtiiPredictedreactivitydicotsincluding:Mesembruanthemumcrystallinum,Solanumtuberosum;monocots(includingAvenasativa,Hordeumvulgare,conifers(Pinusthunbergii),mosses(Physocomitrellapatens),algae(Dunaliellaspp.,Ostreococcusspp.),SaccharomycescerevisiaeNotreactiveinAspergillusnigerAdditionalinformationforadditionalWesternblotdetectionimagepleaserefertothearticlebelow.VERYIMPORTANT:please,donotheatupyoursamplesover70°CasthismightcauseH+ATPasetoprecipitateandtherewillbenosignalonyourwesternblot.SelectedreferencesLietal.(2015).ThreeSAURproteinsSAUR76,SAUR77andSAUR78promoteplantgrowthinArabidopsis.SciRep.2015Jul24;5:12477.doi:10.1038/srep12477.Kolbetal.(2015).FYVE1isessentialforvacuolebiogenesisandintracellulartraffickinginArabidopsisthaliana.PlantPhysiol.2015Feb19.pii:pp.114.253377.Huetal.(2015).Re-examinationofchlorophyllasefunctionimpliesitsinvolvementindefenseagainstchewingherbivores.PlantPhysiol.2015Jan12.pii:pp.114.252023.Komatsuetal.(2014).PhototropinEncodedbyaSingle-CopyGeneMediatesChloroplastPhotorelocationMovementsintheLiverwortMarchantiapolymorphaL.1.PlantPhysiol.2014Sep;166(1):411-27.doi:10.1104/pp.114.245100.Epub2014Aug5.Berthieretal.(2014).Identificationofanewsucrosetransporterinrye-grass(LpSUT2):Effectofdefoliationandputativefructosesensing.PlantPhysiol&Biochem,vol:84,Nov.2014.(immunolocalization,leafsheath)Migockaetal.(2014).MolecularandbiochemicalpropertiesoftwoP1B2-ATPases,CsHMA3andCsHMA4,fromcucumber.PlantCellEnviron.2014Sep11.doi:10.1111/pce.12447.Shenetal.(2014).ThefrondstonoplastquantitativeproteomicanalysisinarsenichyperaccumulatorPterisvittataL.JProteomics.2014Feb4.pii:S1874-3919(14)00047-5.doi:10.1016/j.jprot.2014.01.029.Zhangetal.(2014).ArabidopsisABCG14proteincontrolstheacropetaltranslocationofroot-synthesizedcytokinins.NatCommun.2014Feb11;5:3274.doi:10.1038/ncomms4274.Sobrino-Plataetal.(2014).Glutathioneisakeyantioxidantmetabolitetocopewithmercuryandcadmiumstress.PlantSoil,DOI10.1007/s11104-013-2006-4.applicationexample20µgoftotalproteinfromArabidopsisthaliana(1),Hordeumvulgare(2),Zeamays(3),Nicotianatabaccumplasmamembranefraction,2.5µg(4),extractedwithProteinExtrationBuffer,PEB(AS08300),wereboiledfor10min.in70°Candseparatedon4-12%NuPage(Invitrogen)LDS-PAGEandblotted1htoPVDF.Blotswereblockedimmediatelyfollowingtransferin2%ECLAdvanceblockingreagent(GEHealthcare)in20mMTris,137mMsodiumchloridepH7.6with0.1%(v/v)Tween-20(TBS-T)for1hatroomtemperaturewithagitation.Blotswereincubatedintheprimaryantibodyatadilutionof1:5000for1hatroomtemperaturewithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15minand3timesfor5mininTBS-Tatroomtemperaturewithagitation.Blotswereincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,recommendedsecondaryantibodyAS09602)dilutedto1:20000in2%ECLAdvanceblockingsolutionfor1hatroomtemperaturewithagitation.Theblotswerewashedasaboveanddevelopedfor5minwithECLAdvancedetectionreagentaccordingthemanufacturersinstructions.ImagesoftheblotswereobtainedusingaCCDimager(FluorSMax,Bio-Rad)andQuantityOnesoftware(Bio-Rad).Exposuretimewas2min.immunolocalizationPlasmamembraneH+ATPaselocalizationinArabidopsisthalianaroots.Arabidopsisthaliana,elongationzone,H+ATPase(green).Arabidopsisthalianarootswerefixedinpara-formaldehydefor30minutes.Tissuecleaninghasbeenperformedbeforeimmunolocalization.Anti-rabbitH+ATPase|plasmamembraneprimaryantibodydilutedin1:300andanti-rabbitIgGsecondaryantibodyconjugatedwithAlexa555.Co-stainingwithDAPIvisualizednucleus(bluecolor).Scalebar–100µm.CourtesyDr.TarasPasternak,FreiburgUniversity

Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。