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产品说明
product information
Background
Rabbit anti-goat IgG (H&L), DyLight®350 Conjugated, min. cross-reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight® 350, which binds to Goat IgG (H&L) in immunological assays.DyLight® 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Immunogen
Purified Goat IgG, whole molecule
Host
Rabbit
Clonality
Polyclonal
Clone
Purity
Affinity purified Rabbit IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products
other DyLight® anti-Goat secondary antibodies
Additional information
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with:, heavy (γ) chains on goat IgG, light chains on all goat immunoglobulins
No reactivity is observed to: non-immunoglobulin goat serum proteins, IgG from human, mouse, or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
application information
Recommended dilution
1:20 - 1:2,000 for most applications
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references
to be added when available
Agrisera针对植物蛋白质的保守氨基酸序列,Agirsera还**开发了数十种通用抗体(global antibodies),这类抗体几乎可以与所有的高等植物及藻类产生免疫反应,可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体,Rubisco即核酮糖二磷酸羧化酶,是植物组织中广泛存在的一种高丰度蛋白,在一些植物学WB检测时,经常被用作内参抗体使用。
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