The OKT-3 monoclonal antibody reacts with human CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The OKT-3 antibody has immunosuppressive properties in vivo and has been shown to effectively treat renal, heart and liver allograft rejection. Isotype Mouse IgG2a, κ Recomended Isotype Control(s) InVivoMAb mouse IgG2a isotype control, unknown specificity Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen Not available or unknown Reported Applications in vitro T cell stimulation/activation in vivo T cell depletion in humanized mice ex vivo T cell inhibition for xenografts Flow cytometry Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet