产品说明
The30H12monoclonalantibodyreactswithmouseThy1.2alsoknownasCD90.2.Thy1.2isexpressedbythymocytesandmatureTlymphocytesaswellashematopoieticstemcells,neurons,epithelialcells,andfibroblasts.Thy1.2isexpressedonlybycertainmousestrainsincludingC57BL/6,BALB/c,CBA,C3H,C58/,SJL,DBA,andNZB/.Thy1.2isa25-35kDaGPI-anchoredmembraneglycoproteinandamemberoftheimmunoglobulinsuperfamily.ThefunctionofThy1.2hasnotbeenfullyelucidatedbutisthoughttoplayrolesincognition,axongrowth,Tlymphocytefunction,andapoptosis.The30H12monoclonalantibodyhasbeenreportedtoinduceCa2+fluxinthymocytes.ThisantibodyisparticularlyusefulfordepletionofTlymphocytes.IsotypeRatIgG2bRecomendedIsotypeControl(s)InVivoPlusratIgG2bisotypecontrol,anti-keyholelimpethemocyaninRecommendedInVivoPureDilutionBufferInVivoPurepH7.0DilutionBufferImmunogenMousethymusorspleenReportedApplicationsinvivoILCdepletioninvivoTcelldepletionWesternblotEndotoxin<1EU/mg(<0.001EU/μg)DeterminedbyLALgelclottingassayPurity>95%DeterminedbySDS-PAGEAggregation<5%DeterminedbyDLSFormulationPBS,pH7.0ContainsnostABIlizersorpreservativesSterility0.2μMfilteredProductionPurifiedfromtissueculturesupernatantinananimalfreefacilityPurificationProteinGStorageUndilutedat4°CinthedarkMurinePathogenTestResultsMouseNorovirus:NegativeMouseParvovirus:NegativeMouseMinuteVirus:NegativeMouseHepatitisVirus:NegativeReovirusScreen:NegativeLymphocyticChoriomeningitisvirus:NegativeLactateDehydrogenase-ElevatingVirus:NegativeMouseRotavirus:NegativeTheiler"sMurineEncephalomyelitis:NegativeEctromelia/MousepoxVirus:NegativeHantavirus:NegativePolyomaVirus:NegativeMouseAdenovirus:NegativeSendaiVirus:NegativeMycoplasmaPulmonis:NegativePneumoniaVirusofMice:NegativeMouseCytomegalovirus:NegativeKVirus:NegativeReferencesSDSSheet
Bio X Cell采用最先进的发酵技术;包括搅拌罐发酵罐和波包系统;我们的生产能力从5升到500升不等。
Bio X Cell提供从现有杂交瘤细胞系产生的抗体的生产和纯化服务。这些杂交瘤通常在客户的实验室中开发或在公共领域可用。
该服务包括在细胞培养物中发酵杂交瘤,通过离心收获细胞培养上清液以及通过30 kDa超滤浓缩。通过蛋白A或G亲和色谱进行纯化。将纯化的抗体透析到PBS中并过滤除菌。我们所有定制产品的内毒素水平<2EU / mg。我们还提供冻结细胞系,现场细胞系存储和MAP测试的额外费用。
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