About InVivoMAb anti-mouse PD-L1 (B7-H1)The 10F.9G2 monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1. The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).InVivoMAb anti-mouse PD-L1 (B7-H1) SpecificationsIsotypeRat IgG2b, κRecommended Isotype Control(s)InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(BE0090)Recommended InVivoPure Dilution BufferInVivoPure pH 6.5 Dilution Buffer(IP0065)ImmunogenMouse CD274Reported Applicationsin vivo PD-L1 blockadeImmunofluorescenceImmunohistochemistry (frozen)Flow cytometryWestern blotEndotoxin<2EU/mg (<0.002EU/μg)Determined by LAL gel clotting assayPurity>95%Determined by SDS-PAGEFormulationPBS, pH 6.5Contains no stabilizers or preservativesSterility0.2 μM filteredProductionPurified from tissue culture supernatant in an animal free facilityPurificationProtein GStorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.RRIDAB_10949073Molecular Weight150 kDa