About InVivoPlus anti-mouse IL-10R (CD210)The 1B1.3A monoclonal antibody reacts with mouse IL-10R (IL-10 receptor) also known as CD210. The IL-10R is a class II cytokine receptor and is expressed by a variety of cell types including thymocytes, T lymphocytes, B lymphocytes, NK cells, monocytes, and macrophages. Upon binding IL-10, IL-10R stimulation results in many pleiotropic, effects in immunoregulation and inflammation. IL-10R downregulates the expression of pro-inflammatory cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B lymphocyte survival, proliferation, and antibody production. IL-10R signaling can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. The 1B1.3A antibody is a neutralizing antibody and has been shown to block the binding of human IL-10, which cross-reacts with the mouse IL-10R. However, this clone does not recognize the human IL-10R.InVivoPlus anti-mouse IL-10R (CD210) SpecificationsIsotypeRat IgG1, κRecommended Isotype Control(s)InVivoPlus rat IgG1 isotype control, anti-horseradish peroxidase(BP0088)Recommended InVivoPure Dilution BufferInVivoPure pH 6.5T Dilution Buffer(IPT065)ImmunogenRecombinant ligand-binding domain of mouse IL-10RReported Applicationsin vivo blocking of IL-10/IL-10R signalingin vitro blocking of IL-10R signalingFlow cytometryWestern BlotEndotoxin<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assayBinding ValidationDetermined by immunoblotPurity>95%Determined by SDS-PAGEAggregation<5%Determined by DLSFormulationPBS, pH 6.5 0.01% TweenContains no stabilizers or preservativesSterility0.2 μM filteredProductionPurified from tissue culture supernatant in an animal free facilityPurificationProtein GMolecular Weight150 kDaMurine Pathogen Test ResultsMouse Norovirus: NegativeMouse Parvovirus: NegativeMouse Minute Virus: NegativeMouse Hepatitis Virus: NegativeReovirus Screen: NegativeLymphocytic Choriomeningitis virus: NegativeLactate Dehydrogenase-Elevating Virus: NegativeMouse Rotavirus: NegativeTheiler"s Murine Encephalomyelitis: NegativeEctromelia/Mousepox Virus: NegativeHantavirus: NegativePolyoma Virus: NegativeMouse Adenovirus: NegativeSendai Virus: NegativeMycoplasma Pulmonis: NegativePneumonia Virus of Mice: NegativeMouse Cytomegalovirus: NegativeK Virus: NegativeRRIDAB_1107611StorageThe antibody solution should be stored at the stock concentration at 4°C. Do not freeze.