The 17A2 monoclonal antibody reacts with mouse CD3, a transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3 associates with TCR α/β or γ/δ chains to form the TCR complex. CD3 is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The 145-2C11 antibody has been shown to block the binding of the 17A2 antibody suggests that the 17A2 antibody recognizes an epitope of the CD3ε chain. Treatment with the 17A2 antibody in vivohas been reported to partially deplete T lymphocytes and temporarily down-modulate CD3 expression on T cells. Isotype Rat IgG2b, κ Recomended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen γδ TCR-positive T-T hybridoma D1 Reported Applications in vitro T cell stimulation/activation Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet