The 53-6.7 monoclonal antibody reacts with mouse CD8α. The CD8 antigen is a transmembrane glycoprotein that acts as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. CD8 most commonly exists as a heterodimer composed of one CD8α and one CD8β chain however, it can also exist as a homodimer composed of two CD8α chains. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The molecular weight of each CD8 chain is approximately 34 kDa. The 53-6.7 antibody exhibits depleting activity when used in vivo. Isotype Rat IgG2a, κ Recomended Isotype Control(s) InVivoMAb rat IgG2a isotype control, anti-trinitrophenol Recommended InVivoPure Dilution Buffer InVivoPure pH 6.5 Dilution Buffer Immunogen Mouse Spleen Cells or Thymocyte Membranes Reported Applications in vivo CD8+ T cell depletion Immunofluorescence Flow cytometry Western blot Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 6.5 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet