The M1/70 monoclonal antibody reacts with mouse and human CD11b, a 170 kDa membrane glycoprotein also known as integrin alpha M (ITGAM). CD11b belongs to the integrin alpha family and is primarily expressed on granulocytes and monocytes/macrophages but also expressed on dendritic cells, NK cells, and subsets of T and B cells. CD11b and CD18 combine to form Mac-1. Mac-1 functions as a complement receptor as well as a receptor for fibrinogen, factor X, and ICAM1. Isotype Rat IgG2b, κ Recomended Isotype Control(s) InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen B10 mouse spleen cells enriched for T cells Reported Applications in vivo CD11b neutralization ILC2 cell purification Flow cytometry Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet