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BioX cell/InVivoMAb anti-mouse CD22/BE0011/25MG
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产品说明
The Cy34.1 monoclonal antibody reacts with mouse CD22, a member of the SIGLEC family of lectins. CD22 is expressed at high levels on the surface of mature follicular and marginal zone B lymphocytes, B-1 cells, and plasma cells and associates with the B-cell antigen receptor. CD22 mediates B cell adhesion to ligands on endothelial cells in the bone marrow. Additionally, CD22 to negatively regulates B cell activation and prevents the development of autoimmune diseases. The Cy34.1 antibody has been shown to augment B cell proliferation in response to LPS or anti-mouse Ig µ chain. Isotype Mouse IgG1, κ Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen B10.D2 mouse splenocytes Reported Applications in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κ Light Chain (clone MAR 18.5) Flow cytometry Immunoprecipitation Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet

Bio X Cell采用最先进的发酵技术;包括搅拌罐发酵罐和波包系统;我们的生产能力从5升到500升不等。

Bio X Cell提供从现有杂交瘤细胞系产生的抗体的生产和纯化服务。这些杂交瘤通常在客户的实验室中开发或在公共领域可用。

该服务包括在细胞培养物中发酵杂交瘤,通过离心收获细胞培养上清液以及通过30 kDa超滤浓缩。通过蛋白A或G亲和色谱进行纯化。将纯化的抗体透析到PBS中并过滤除菌。我们所有定制产品的内毒素水平<2EU / mg。我们还提供冻结细胞系,现场细胞系存储和MAP测试的额外费用。


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