The Cy34.1 monoclonal antibody reacts with mouse CD22, a member of the SIGLEC family of lectins. CD22 is expressed at high levels on the surface of mature follicular and marginal zone B lymphocytes, B-1 cells, and plasma cells and associates with the B-cell antigen receptor. CD22 mediates B cell adhesion to ligands on endothelial cells in the bone marrow. Additionally, CD22 to negatively regulates B cell activation and prevents the development of autoimmune diseases. The Cy34.1 antibody has been shown to augment B cell proliferation in response to LPS or anti-mouse Ig µ chain. Isotype Mouse IgG1, κ Recomended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen B10.D2 mouse splenocytes Reported Applications in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κ Light Chain (clone MAR 18.5) Flow cytometry Immunoprecipitation Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification Protein G Storage Undiluted at 4°C in the dark References SDS Sheet