The 7D4 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The 7D4 antibody recognizes a different epitope on CD25 than the PC-61.5.3 clone. Isotype Rat IgM, κ Recomended Isotype Control(s) InVivoMAb polyclonal rat IgG Recommended InVivoPure Dilution Buffer InVivoPure pH 7.0 Dilution Buffer Immunogen IL-2-dependent BALB/c mouse T cell clone HT2 Reported Applications in vivo regulatory T cell inactivation Endotoxin <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay Purity >95% Determined by SDS-PAGE Formulation PBS, pH 7.0 Contains no stabilizers or preservatives Sterility 0.2 μM filtered Production Purified from tissue culture supernatant in an animal free facility Purification T-Gel Storage Undiluted at 4°C in the dark References SDS Sheet