Cat #: ABR-022 Alternative Name Brain-specific angiogenesis inhibitor 2, Adhesion G protein-coupled receptor B2, ADGRB2Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: h, m, r Immunogen - + Peptide (C)EPKVKTQWPRSADE, corresponding to amino acid residues 277-290 of mouse BAI2 (Accession Q8CGM1). Extracellular, N-terminus.Accession (Uniprot) Number Q8CGM1Gene ID 230775Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Rat, human - identical. RRID AB_2756544.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.Antibody concentration after reconstitution 0.8 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 µg peptide per 1 µg antibody.Standard quality control of each lot Western blot analysis.Applications: wbMay also work in: ic*, ifc*, ih*, ip*, lci* Western blot - + Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-BAI2 (extracellular) Antibody (#ABR-022), (1:200).3,4. Anti-BAI2 (extracellular) Antibody, preincubated with the negative control antigen.Western blot analysis of human SHSY-5Y neuroblastoma cell line lysates:1. Anti-BAI2 (extracellular) Antibody (#ABR-022), (1:200).2. Anti-BAI2 (extracellular) Antibody, preincubated with the negative control antigen. References1. Okajima, D. et al. (2009) J. Recept. Signal Transduct. Res. 30, 143.2. Jeong, B.C. et al. (2006) FEBS Lett. 580, 669. Scientific background - + Brain-specific angiogenesis inhibitor 2 (BAI2) is a member of the adhesion G-protein coupled receptor (GPCR) family. BAI2, and other members of its family, commonly have a long N-terminal extracellular region (ECR) containing a GPCR proteolysis site (GPS) adjacent to the seven-transmembrane (7TM) domain. The long N-terminus contains common structural domains, including thrombospondin type-I repeats (TSRs), epidermal growth factor (EGF)-like, leucine-rich repeats, lectin-like, immunoglobulin (Ig), and cadherins. BAI2 is composed of 521 amino acids and is mainly expressed in neurons.Although BAI2 is a GPCR, there are currently no reports showing evidence of any physiological ligands. Instead, BAI2 is activated by cleavage and dissociation of the ECR from its 7TM fragment, and functions as a GPCR. BAI2 is glycosylated and cleaved at the GPS by furin in the ER. The cleaved N-terminal ECR associates with the C-terminal 7TM fragment and they are translocated to the cell surface. The ECR associated with the 7TM fragment interacts with other membrane proteins or matrix proteins through the TSRs and dissociates from the 7TM fragment. The 7TM fragment which has released the associated ECR changes its conformation into the active state and activates the G protein-coupled signaling1.BAI2 is involved in the early stages of neovascularization of cerebral cortex after ischemia. Decreased levels of BAI2 induce increased VEGF expression mediated by GA-binding protein gamma (GABPγ) in cerebral ischemia and under normal conditions2.Application key:CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry,IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen IncludedClick here to receivea 25 µl free trial sample!Certificate of Analysis MSDSLast update: 17/10/2019Anti-BAI2 (extracellular) Antibody (#ABR-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize BAI2 from rat, mouse, and human samples.For research purposes only, not for human use