首页 > Discovery > Discovery Antibodies/Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody/50 µl/ACC-021-AR-50 µl

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Discovery Antibodies/Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody/50 µl/ACC-021-AR-50 µl

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ACC-021-AR-50µl

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Discovery

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Cat#:ACC-063-AOAlternativeNameStromalinteractionmolecule1,GOKLyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!Type:PolyclonalHost:RabbitReactivity:h,m,rImmunogen-+PeptideCHSEDEKLSFEAVR,correspondingtoaminoacidresidues56-69ofhumanSTIM1(Accession Q13586). STIM1expressedontheplasmamembrane:Extracellular,N-terminus.STIM1expressedontheER:Luminal,N-terminus.Accession(Uniprot)NumberQ13586GeneID6786PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,mouse,bovineandcanis-identical.RRIDAB_2039894.PurityAffinitypurifiedonimmobilizedantigen.FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.IsotypeRabbitIgG.LabelATTO-550.Maximumabsorption554nm;maximumfluorescence576nm.Thefluorescenceisexcitedmostefficientlyinthe540-565nmrange.ThislabelisrelatedtothedyeRhodamine6GandcanbeusedwithfiltersusedtodetectRhodamine.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50µldoubledistilledwater(DDW).Antibodyconcentrationafterreconstitution1mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).Negativecontrolantigenstorageafterreconstitution-20°C.PreadsorptionControl10μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#ACC-063),andimmunohistochemistry(labeledantibody).Applications:ic,if,ih,lciImmunohistochemistry-+Expression ofSTIM1inratpancreasImmunohistochemicalstainingofratparaffin-embeddedpancreassectionsusingAnti-STIM1(extracellular)-ATTO-550 Antibody(#ACC-063-AO),(1:20),(red).A.STIM1stainingishighlyspecificforendocrinecellsintheIsleofLangerhans.B.Hoechst33342(blue)isusedasthecounterstain.C.MergedimageofpanelsAandB.Livecellimaging/Immunocytochemistry-+ExpressionofSTIM1inRBLcellsCellsurfacedetectionofSTIM1inliveRBLcells.A.ExtracellularstainingofcellswithAnti-STIM1(extracellular)-ATTO-550 Antibody(#ACC-063-AO),(1:20).B.Nuclearstainingofcellsusingthecell-permeabledyeHoechst33342.C.MergedimageofpanelsAandB.References1.Eisner,D.A.etal.(2005)Exp.Physiol.90,3.2.Chakrabarti,R.andChakrabarti,R.(2006)J.Cell.Biochem.99,1503.3.Feske,S.etal.(2006)Nature441,179.4.Pickett,J.(2006)NatureReviewsMol.CellBiol.7,794.5.Dziadek,M.A.andJohnstone,L.S.(2007)CellCalcium42,123.6.Yuan,J.P.etal.(2007)Nat.CellBiol.9,636.7.Muik,M.etal.(2008)J.Biol.Chem.283,8014.8.Li,Z.etal(2007)J.Biol.Chem.282,29448.9.Hewavitharana,T.etal.(2007)CellCalcium42,173.Scientificbackground-+CytosolicCa2+ haslongbeenknowntoactasakeysecondmessengerinmanyintracellularpathwaysincludingsynaptictransmission,musclecontraction,hormonalsecretion,andcellgrowthandproliferation.1,2 ThemechanismcontrollingtheinfluxofintracellularCa2+ eitherbycalcium-release-activatedCa2+ channels(CRAC)orfromintracellularstoreshaslatelybecomeofgreatinterest.Recently,severalkeyplayersofthestore-operatedcomplexhavebeenidentified.3 TheOraifamilyconsistsofthreemembers,Orai1-3,andtheSTIMfamily,whichconsistsoftwomembers,STIM1andSTIM2.Orai1(alsoknownasCRACM1)actsasthestore-operatedcalciumchannel(SOC)andSTIM1astheendoplasmicreticulum(ER)Ca2+ sensor.3,4 ThemajorityofSTIM1appearstobelocalizedintracellularlyattheERmembranewhilelowexpressionofSTIM1hasbeendetectedonthecellsurfaceofseveralcelltypes.5 STIM1hasanamino-terminalEFhandCa2+ bindingdomainfacingthelumenoftheER.6 UponCa2+ storedepletion,STIM1moleculesareredistributedinpunctaeunderneaththeplasmamembraneandactivateSOCs.SeveralpossIBLeinteractionsbetweenSTIM1andOrai1havebeensuggested.ThemostsimpleandcitedisadynamicinteractionbetweenthecytosolicC-terminusofSTIM1andthecytoplasmicdomainoftheOrai1channel.7-9 STIM1isassumedtoregulatetheactivityofallknownSOCs,includingnativeSOCs.5 ConsistentwiththeirimportantroleascalciumsensorswithintheER,STIM1proteinsareubiquitouslyexpressed.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderNegativeControlAntigenIncludedCertificateofAnalysisMSDSLastupdate:10/11/2019Anti-STIM1(extracellular)Antibody(#ACC-063)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanStromalinteractionmolecule1.Theantibodycanbeusedinwesternblot,immunocytochemistry,immunohistochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeSTIM1fromhuman,rat,andmousesamples.Anti-STIM1(extracellular)-ATTO-550Antibody(#ACC-063-AO)isdirectlylabeledwithanATTO-550fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-550fluorescentlabelisrelatedtothewellknowndyeRhodamine6GandcanbeusedwithfilterstypicallyusedtodetectRhodamine.Anti-STIM1(extracellular)-ATTO-550AntibodyhasbeentestedinimmunocytochemistryandimmunohistochemistryapplicationsandisespeciallysuitedforexperimentsrequiringsimultaneouslabelingofdifferentMarkers.Forresearchpurposesonly,notforhumanuse

Discovery®抗体是剑桥生物化学研究所（CRB）的新目录抗体业务。

CRB是世界上第二大多肽公司，也是抗体研究市场的领跑者，从目录业务到现在已有22年的休息期（1994-2016年），现在是第37年（2017年），CRB推出了一个全新的现成抗体业务，名为Discovery®抗体。

Discovery®抗体将专注于研究抗体（早期药物“Discovery”），并将补充我们非常成功和长期的定制抗体业务(www.crbdiscovery.com网站). 我们以新颖、复杂、稀有和翻译后修饰的特异性抗体而闻名，这些备受追捧的目标将出现在我们的发现®抗体目录范围内。

我们于2017年2月推出了我们的发现®抗体，一年后发现®肽，大约240年后，詹姆斯·库克船长（他那个时代最伟大的探险家）于1776年从英国乘“发现”号船出发，包租全球的新水域和“发现”新大陆。因此，“发现”这个名字似乎适合东北部的一家制造商——詹姆斯·库克的家乡。

在17世纪末对世界各地新发现的土地的探索和2000年医学研究的新“发现”以及人类基因组的绘制之间产生了进一步的相似之处。

有关更多详情，请参阅我们发布的Discovery®抗体的新闻稿

剑桥研究生物化学(www.crbdiscovery.com网站)是一家领先的定制肽和抗体工具的独立生产商，为全球制药、生命科学和学术领域的研究人员提供服务。其产品的主要用途是作为定制的实验室级试剂，用于证明早期药物发现中的主要研究，并支持临床研究中的生物标记物方案。该公司生产各种口味的肽，从简单到改性，稳定，从毫克到克标到最高纯度的染料标签。

Discovery®抗体是剑桥生物化学研究所（CRB）的新抗体目录业务