首页 > Discovery > Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody/50 µl/APC-101-B-50 µl

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Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody/50 µl/APC-101-B-50 µl

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APC-101-B-50µl

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Discovery

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Cat#:APC-101-BAlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!Type:PolyclonalHost:RabbitReactivity:h,m,rImmunogen-+PeptideKDYPASTSQDSFEA(C),correspondingtoaminoacidresidues263-276ofhumanKV1.3(AccessionP22001).ExtracellularloopbetweendomainsS1andS2.Accession(Uniprot)NumberP22001GeneID3738PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,mouse -12/14aminoacidresiduesidentical.RRIDAB_2341030.PurityAffinitypurifiedonimmobilizedantigen.FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.IsotypeRabbitIgG.LabelBiotin.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50µldoubledistilledwater(DDW).Antibodyconcentrationafterreconstitution1mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).Negativecontrolantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:fc,if,ih,wbMayalsoworkin:ic*,ip*,lci*Westernblot-+WesternblotanalysisofhumanJurkatTcellleukemiacelllysate(lanes1and3)andmousebrainlysate(lanes2and4):1-2. Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B),(1:1000).3-4.Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody,preincubatedwiththecontrolpeptideantigen.Immunohistochemistry-+Immuno-colocalizationofKV1.3andKV1.5inmousecerebellumImmunohistochemicalstainingofmouseperfusion-fixedfrozenbrainsectionsusing Anti-KV1.3(KCNA3)(extracellular)-Biotin Antibody(#APC-101-B)(1:400),and Anti-KV1.5(KCNA5)-ATTO-550Antibody (#APC-004-AO),(1:60).A.KV1.3staining(green)isdetectedinBergmanngliasomaandprocessesinthemolecularlayer(Mol)(arrow).B.SamesectionshowsstainingforKV1.5(red).C.Mergeofthetwoimagessuggestsconsiderableco-localizationinthesomaofBergmannglia.Inthemolecularlayer,thedistributionofKV1.5isdiffuse,unlikethediscretestainingforKV1.3inglialprocesses.CellnucleiwerevisualizedwithDAPI(blue).Directflowcytometry-+CellsurfacedetectionofKV1.3inliveintacthumanJurkat Tcellleukemiacellline:___ Cells+Streptavidin-Alexa647.___ Cells+ Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B),(1:10)+Streptavidin-Alexa647.Thecontrolantigenisnotsuitableforthisapplication.References1.Chandy,K.G.etal.(2001)Toxicon39,1269.2.Koo,G.C.etal.(1997)J.Immunol.158,5120.Scientificbackground-+KV1.3belongstothe Shaker familyofvoltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1ThecentralroleofKV1.3inimmunesystemcellscreatedarealneedforaspecific antibodythatwouldbeabletoworkinflowcytometryapplications.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderNegativeControlAntigenIncludedCertificateofAnalysisMSDSLastupdate:16/01/2020Anti-KV1.3(KCNA3)(extracellular)Antibody(#APC-101)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,mouse,andratsamples.Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody(#APC-101-B)isdirectlylabeledwithbiotin.StrepavidintaggedwithHRPorwithafluorescentprobecanthenbeusedtodetecttheprotein.Thebiotin/strepavidinsystemisidealforminimizingcross-reactivitywhensamespeciesantibodiesaresimultaneouslyused.Anti-KV1.3(KCNA3)(extracellular)-BiotinAntibody hasbeentestedinwesternblot,immunohistochemistryanddirectflowcytometryandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.Forresearchpurposesonly,notforhumanuse

Discovery®抗体是剑桥生物化学研究所（CRB）的新目录抗体业务。

CRB是世界上第二大多肽公司，也是抗体研究市场的领跑者，从目录业务到现在已有22年的休息期（1994-2016年），现在是第37年（2017年），CRB推出了一个全新的现成抗体业务，名为Discovery®抗体。

Discovery®抗体将专注于研究抗体（早期药物“Discovery”），并将补充我们非常成功和长期的定制抗体业务(www.crbdiscovery.com网站). 我们以新颖、复杂、稀有和翻译后修饰的特异性抗体而闻名，这些备受追捧的目标将出现在我们的发现®抗体目录范围内。

我们于2017年2月推出了我们的发现®抗体，一年后发现®肽，大约240年后，詹姆斯·库克船长（他那个时代最伟大的探险家）于1776年从英国乘“发现”号船出发，包租全球的新水域和“发现”新大陆。因此，“发现”这个名字似乎适合东北部的一家制造商——詹姆斯·库克的家乡。

在17世纪末对世界各地新发现的土地的探索和2000年医学研究的新“发现”以及人类基因组的绘制之间产生了进一步的相似之处。

有关更多详情，请参阅我们发布的Discovery®抗体的新闻稿

剑桥研究生物化学(www.crbdiscovery.com网站)是一家领先的定制肽和抗体工具的独立生产商，为全球制药、生命科学和学术领域的研究人员提供服务。其产品的主要用途是作为定制的实验室级试剂，用于证明早期药物发现中的主要研究，并支持临床研究中的生物标记物方案。该公司生产各种口味的肽，从简单到改性，稳定，从毫克到克标到最高纯度的染料标签。

Discovery®抗体是剑桥生物化学研究所（CRB）的新抗体目录业务