首页 > Lucigen > Lucigen/TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105/TAB1R80524/24 Reactions

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Lucigen/TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105/TAB1R80524/24 Reactions

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Optimizedforhighyield,highqualitybiotin-aRNAHighlyreproducIBLeamplification,labeling,andmicroarrayresults.MultipleRNAsamplescanbeeasilyamplifiedandlabeledsimultaneously.LinearRNAamplificationprocesspreservesthetranscriptprofileofthesample.Simple,single-tube,6-hourprocedure.Preparebiotin-aRNAandbeginhybridizationthesameday.Biotin-aRNAproducedbythekitdetectsmoretranscripts(genes)thancompetitorkitsandshowedhighcorrelationwithMAQCTaqMan®data(Fig.1).ApplicationsTheTargetAmp™1-RoundBiotin-aRNAAmplificationKit105*producesmicrogramamountsofbiotin-antisenseRNA(biotin-aRNA,alsocalledbiotin-cRNA)fromaslittleas25ngoftotalcellularRNA.Thekitprovidesallreagentsforthepreparationofbiotin-aRNA(exceptreversetranscriptaseandRNApurificationcolumns)andusesanimprovedEberwine-typelinearamplificationmethod1toachieveatleast5,000-foldamplificationofthepoly(A)RNApresentinatotalcellularRNAsample.Theentireprocedurecanbecompletedin1day.Table1.ATargetAmp™Kit105reactionproduceshighyieldsofbiotin-aRNAfromaslittleas25ngofinputtotalRNA.Resultsrepresenttheaverageofmultipleexperiments.AmountofInputHeLaTotalRNABiotin-aRNAYieldfromHeLaTotalRNAa25ng4.1µg100ng13.7µg500ng64.9µga.Approximately2%ofHeLatotalRNAispoly(A)RNA.Figure1.CorrelationofexpressionratiosobtainedfromqPCRandfrommicroarrayshybridizedtoaRNApreparedwiththeTargetAmp™105kit.Xaxis,log2expressionratiosdeterminedfromHumanGenomeU133Plus2.0Arrays(Affymetrix)hybridizedtotargetspreparedbytheTargetAmp105kit;Yaxis,log2expressionratiosdeterminedbytheMAQC,usingTaqMan®assays.Thehighcorrelationobtained(r=0.936)indicatestheexcellentfidelityoftheamplificationprocess.ReferencesVanGelder,R.N.etal.(1990)Proc.Natl.Acad.Sci.USA87,1663.*TargetAmp™aRNAAmplificationProductsarecoveredbyintellectualpropertylicensedtoEpicentreTechnologiesCorporationfromJohnson&JohnsonPharmaceuticalResearch&Development,L.L.C.,andbyintellectualpropertysublicensedtoEpicentreTechnologiesCorporationfromIncyteCorporation.Forresearchuseonly.Someapplicationsinwhichthisproductmaybeusedmaybecoveredbypatentsorpatentapplicationsapplicableincertaincountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainalicense,dependingupontheparticularapplicationandcountryinwhichtheproductisused.ORDERINFORMATIONThesekitswereoptimizedusingSuperScript™IIIreversetranscriptase(Invitrogen),whichisnotincludedinthekits.Contents:TargetAmp™T7-Oligo(dT)PrimerA,TargetAmp™ReverseTranscriptionPreMix,TargetAmp™DNAPolymerasePreMixI,TargetAmp™DNAPolymerase,TargetAmp™T7RNAPolymerase,TargetAmp™T7TranscriptionBuffer,TargetAmp™NTPPreMix,TargetAmp™UTP/Biotin-UTP,RNase-FreeDNaseI,DTT,HeLaTotalRNAControl,RNase-FreeWater.

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