首页 > Lucigen > Lucigen/EconoTaq PLUS GREEN 2X Master Mix/30033-2/1,000 rxns

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Lucigen/EconoTaq PLUS GREEN 2X Master Mix/30033-2/1,000 rxns

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30033-2

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4000-520-616

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OutstandingperformanceandvalueareperfectforroutinePCR.Canbecycledupto98°CtoamplifythemostchallengingGC-richtemplatesotherscannot.Ready-to-usePCRreactionmastermixes,containdNTPsandPCREnhancer.Non-proofreADIngpolymeraseEconoTaqPLUSGREENcontainstrackingdyesforgelelectrophoresisFreeSampleAvailable EconoTaq®PLUSGREEN&EconoTaqPLUS2XMasterMixesofferPerformance,Convenience,ReliABIlity,andValueforRoutinePCR.MoreeffectivethanotherTaqmastermixes.EconoTaqPLUSandPLUSGREEN2XMasterMixescontainaproprietaryPCRenhancer,sotheycansuccessfullyamplifytemplatesthatfailtoamplifywithotherPCRmastermixes(Figure1).HighdensityreactionbufferenablesdirectgelloadingfollowingPCR.ExcellentresultsinroutinePCR.EconoTaqPLUSGREEN&EconoTaqPLUScontainhighpurity,highactivityEconoTaqDNAPolymeraseforreliableamplificationoftemplatesupto5kb(Figure1).AmplifychallengingGC-richtemplates.These2XMasterMixescanbecycledupto98°CtoamplifychallengingGC-richtemplatesotherscannot(Figure2).ConvenienttrackingdyesinEconoTaqPLUSGREEN.Ona1%agarosegel,thebluedyemigratesatthesamerateasa5kbDNAfragment,andtheyellowdyemigratesat75bp.SeeFigure3.Ifdesired,thedyesareeasilyremovedbystandardDNApurificationproductsorethanolprecipitation.Forfluorescenceorabsorbancedetectionassays,EconoTaqPLUS2XMasterMixoffersthesamegreatperformancewithoutthedyes.FlexIBLe&easytouse.EconoTaqPLUSGREENandEconoTaqPLUSarepackagedin50reactionvialsthatcanbestoredintherefrigerator(+4ºC)forupto3months.Theyarestableforatleast10freeze-thawcycles.Lowprice!EconoTaqPLUSGREENisonly26¢per50µlPCRreaction…foreverything!EconoTaqPLUSGREEN&EconoTaqPLUSoffergreatvaluewhencomparedtoTaqmastermixes. EconoTaqPLUSGREEN&EconoTaqPLUSprices(see“OrderingandPricing”)arebasedona50µlPCR;usinga25µlreactionvolumewillstretchyourresearchbudgetevenfurther. Figure1.EconoTaqPLUSGREENMasterMixvs.GoTaq®Greenmastermix(Promega)inPCR.PCRwasperformedaccordingtothemanufacturer’srecommendationsusingprimersspecificfortheindicatedgenes.PanelA,0.7kbprokaryoticsingle-strandedDNAbindingprotein(genomicDNA);PanelB,2.7kbprokaryoticDNApolymeraseA(genomicDNA);PanelC,4.5kbgene(plasmidDNA).Figure2.GC-richregionsfromhumangenomicDNAwerePCRamplifiedusing98°Cdenaturation.Figure3.EasyvisualizationwithEconoTaqPLUSGREEN.Duringelectrophoresis,thegreenloadingcolorseparatesintoaslow-movingbluedyeandafast-movingyellowdye.Legal:PleaseNote:SomeapplicationsinwhichLucigen'sEconoTaqPLUSGREENandEconoTaqPLUS2XMasterMixescanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.ORDERINFORMATIONUseEconoTaqPLUSGREEN2XMasterMixwhenthePCRproductswillbeanalyzedbyagarosegelelectrophoresisandethidiumbromidestaining.UseEconoTaqPLUS2XMasterMixwhenthePCRproductswillbeanalyzedbyabsorbanceorfluorescenceexcitation,toavoidpossibleinterferencebythedyes.EconoTaqPLUSGREEN&EconoTaqPLUSpricesarebasedona50µlPCRreaction.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。