首页 > Lucigen > Lucigen/CloneID™ 1X Colony PCR Mix/30059-2/500 rxns

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Lucigen/CloneID™ 1X Colony PCR Mix/30059-2/500 rxns

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30059-2

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OutstandingreliABIlityandreproducibilityinColonyPCRIdealforPCRgenotypingConvenient1XMasterMix:justaddacolony,primers,andcycle.HighThermostabilityandoptimizedformulationfor98Cdenaturationtohandlethetoughesttargets.ContainsGreentrackingdyefordirectgelloADIngFreeSampleAvailable Finally,aPCRenzymespecificallyformulatedforColonyPCR.Reliableperformancefromcolonies.Bypassallcelllysisandsampleprepwithoutworryingaboutfailedreactions.Outstandingreproducibilitytogetyourampliconthefirsttime.(Figure1).Extremethermostability. Cycleat98°Ctolysecellsanddenaturethetoughesttemplates.TheaddedPCRenhancerimprovesamplificationandthermostabilitytogiveyouthebestPCRproductpossIBLe.Toughtemplatesarenomatch: GC-richDNAcanmakecolonyPCRthatmuchmore difficult–andCloneIDcanhandleitwithease.Cleanandrobustamplificationontargetswhereotherenzymesfallshort(Figure2)Theeasiestworkflow. CloneIDisnotonlya1Xmastermixthatonlyrequiresacolonyswipeandprimers,butitalsocontainsaGreentrackingdyesoyoucandirectlyloadyourPCRproductontoagel.Figure1.CloneID™1XColonyPCRMasterMixwasusedon96uniquecoloniesfromapetridishcontainingalibrarysample.Successratewas97%(93outof96)foramplification..Figure2.CloneID™1XColonyPCRMasterMixiscomparedwithotherPCRproducts(CloneID™,Platinum®PCRSupermix,OneTaq®,KAPA2G)claimingstrongColonyPCRperformance.The12targetsaregenesfromCellulomonasfimi,aspecieswhosegenomeis75%GC,whichwereclonedintoastandardplasmid.ORDERINFORMATIONCloneID™1XColonyPCRMasterMixisprovidedina1XMasterMixformatthatwithstands98°Cdenaturation.Allrequiredbuffercomponents,enzyme,Mg++anddNTP’sareallpresentinoptimizedconcentrations.TheproductalsocontainsGreentrackingdyeforconvenientgelloading.Onlyasmallamountofwholecellsfromacolonyandprimersarerequiredtosetupthereaction.Pleaseseetheusermanualforcompositioninformation.Standardreactionsizeis25µL.ThisproductisprimarilyintendedforE.colicoloniesorpurifiedDNAtemplates.CloneIDhasnotbeentestedfordiverseorganisms.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。