产品说明
PerfectforantibodyphagedisplayorpeptidephagedisplaylibrarycreationThehighestefficiencyTG1competentcellsavailable:≥4×1010cfu/µg.YouronlysourceforelectrocompetentSS320andER2738cellsAmbersuppressorandnon-ambersuppressorlinesformaximumflexibilityHighefficiencyMC1061F-cellsforcloningorcontrolreactionsCreatelargerlibraries;speeddiscoveryBulkcustomdispensingavailablewithallofourphagedisplaycellsFreeSampleAvailable MC1061F-ElectrocompetentCells.Anon-ambersuppressorstrain,identicaltoSS320exceptthatitlackstheF′episome.Highlyefficient(≥4×1010cfu/µg)electrocompetentcellsforgeneralcloningorPhageDisplay.NotethatasanF-strain,itcannotbeusedforre-infectionbyfilamentousphage. Thisstrainnotincludedinthesamplekit.TG1ElectrocompetentCells.Anambersuppressorstrain(supE)preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellsareusedforphagedisplayandproteinexpression.SS320ElectrocompetentCells.Anon-ambersuppressorstrain(sometimescalledMC1061F')preparedashighlyefficient(≥4×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.Thesecellshavethehighestavailabletransformationefficiencyofanystrainusedforphagedisplay.ER2738ElectrocompetentCells.Anambersuppressorstrain(glnV)preparedashighlyefficient(≥2×1010cfu/µg)electrocompetentcellsforphagedisplaylibraryscreening.ThisstrainisrecommendedforusewithNewEnglandBiolab'sPh.D.™PhageDisplayKits.Figure1.TransformationefficiencyofLucigen'selectrocompetentbacterialcellsforphagedisplaycomparedtocompetitor'sspecification.Table1.CompetentCellsforPhageDisplaylibrariesCellLinesTransformationEfficiency(cfu/µgpUCDNA)CloningMethylatedDNABAC,CosmidCloningBlue/WhiteScreeningTG1Electrocompetent>4×1010YESNOYESIPTGinductionrequiredSS320(MC1061F')Electrocompetent>4×1010YESNOYESIPTGinductionrequiredMC1061F-Electrocompetent>4×1010YESNOYESIPTGinductionrequiredER2738Electrocompetent>2×1010YESNOYESIPTGinductionrequiredCustomCompetentCellsBulkLucigencellsoryourstrainofE.coli,madetothehighestpossIBLetransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellsfordetailedinformation.ORDERINFORMATIONCompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasDUOs(2transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。
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