产品说明
GeneralcloningstrainClonelargeinsertsandtransformlargeplasmids-uptoatleast145kbplasmidDNA.Achievehighefficiencieswithconvenientchemicallycompetentformat:>5×108cfu/µgpUC19DNA.Idealforcloning,subcloningandplasmidpreparationduetoendAandrecAmutations.ApplicationsRoutinecloningofDNAupto200kb.ThehighlyversatileTransforMax™EC100™E.colicompetentcellsareidealformostcloningapplications.ThecellsprovideveryhightransformationefficiencywhentestedagainstawiderangeofsupercoiledDNAsaswellasDNAdirectlyfromaligationreaction(Table1).BenefitsHightransformationefficiencywithclonesofallsizes,includingBACclones(Table 1).lacZΔM15forblue/whitescreeningofrecombinants.Restrictionminus[mcrA,Δ(mrr-hsdRMS-mcrBC)]enablesefficientcloningofmethylatedDNA.Endonucleaseminus(endA1)toensurehighyieldsofDNA.Recombinationminus(recA1)forgreaterstABIlityoflargeclonedinserts.DNATransforMax™EC100™ChemicallyCompetentE. coliTransforMax™EC100™ElectrocompetentE. colipUC191.4×1081.4×10108.1-kbClone1.3×107Nottested13.1-kbClone4.3×1061.3×10923.1-kbClone9.2×1053.0×108145-kbBACCloneNottested7×10713.1-kbclonedirectlyfromaligationreaction2.2×1052.1×107Table1.ComparisonofthetransformationefficienciesofTransforMax™EC100™E. coliwithavarietyofDNAs.Transformationswereperformedusing50µlofcompetentcellsandeithersupercoiledDNAsoftheindicatedsizesora1-µlaliquotfromastandard10-µlligationreaction.Resultsshownareincfu/µgofDNAandaretheaveragetransformationefficienciesobtainedfromseveraltrials.GenotypeF-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalKλ-rpsL(StrR)nupGTransforMaxEC100ElectrocompetentE.coliTransformationefficiencyof>1×1010cfu/µgofpUC19.TransforMaxEC100ChemicallyCompetentE.coliTransformationefficiencyof>5×108cfu/µgofpUC19.ORDERINFORMATIONIncludespUC19controlDNA.
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。
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Biosearch technologies lucigen感受态细胞选择指南_北京中北林格...
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Lucigen/EconoTaq DNA Polymerase (separate Mg++)/30032-2/5,000 U (5 x 1,000 U)
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Lucigen/DuraScribe® T7 Transcription Kit/DS010925/25 Reactions
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Lucigen/EconoTaq DNA Polymerase (with Mg++)/30031-2/5,000 U (5 x 1,000 U)
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Lucigen/Expresso® CMV Cloning & Expression System/49031-1/5 rxns
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Lucigen/DNATerminator® End Repair Kit/40035-2/50 rxns
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Lucigen 中国代理_化工仪器网
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Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/60052-1/12 rxns (DUOs)
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Lucigen/FailSafe™ PCR 2X PreMix L/FSP995L/2.5 ml