产品说明
GreatTaqPolymeraseperformanceatalowprice.Choiceofreactionbuffers,withorwithoutMgCl2.Non-proofreADIngPolymeraseFreeSampleAvailable At$96for1000unitslistprice(quantitydiscountsavailable),EconoTaq’slowpriceiscoupledwithhighqualityandperformance.QCspecificationsforEconoTaqarerigorous:Greaterthan99%purebySDSgelelectrophoresis(seeFigure1).NodetectableDNAcontaminationasdeterminedbyPCRusinggenericprimers.Nodetectableendonuclease(nicking)activity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofsupercoiledpBR322DNAfor16hoursat70°Cresultsinnodetectableconversiontorelaxedorlinearformsbyagarosegelelectrophoresis.Nodetectableexonucleaseactivity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofHindIII-cutlamBDaDNAfor16hoursat70°Cresultsinnosmearingofbandsonagarosegels.EconoTaqPerformanceAsshowninFigures2and3Lucigen’sEconoTaqDNAPolymeraseperformsaswellas,orbetterthan,moreexpensiveTaqpreparationsfromseveralothersuppliersinroutinePCR.EconoTaqisaseffectiveinDNAamplificationasanotherstandardPCRenzyme,TflDNApolymerase(Figure4).Inthiscase,thebackgroundofnon-specificamplificationwasmuchlowerwithEconoTaq(compare“+”and“–“lanesforEconoTaqandTflinFigure4).EconoTaqDNAPolymerasealsooffershighlot-to-lotreproducibilityandreliABIlity(Figures2and4)Figure1.HighpurityofEconoTaqDNAPolymerase(SDSPAGE).Lane1,broadrangemolecularweightMarkers;Lane2,LucigenEconoTaqDNAPolymeraseFigure2.TaqDNApolymerasefromPromegaandNewEnglandBiolabswerecomparedtoLucigen’sEconoTaqDNAPolymerase(2differentlots)inamplifyingtheampicillingene(0.8kb)inapUC19vector.(–),noDNA.(+),DNAadded(40ng).MW,1kbladder.Figure3.EconoTaqvs.AmpliTaq®(AppliedBiosystems)DNApolymeraseingenotyping.AllPCRreactionswereperformedinaRoboCycler96(Stratagene).Hip1genotypingwasperformedusingthefollowingPCRconditions:94°Cfor1min,35cyclesof94°Cfor30sec,62°Cfor60sec,72°Cfor90sec,and72°Cfor7min.Shh,CdoandGas1genotypingwereperformedusingthefollowingPCRconditions:94°Cfor2min,35cyclesof94°Cfor60sec,65°Cfor60sec,72°Cfor90sec,and72°Cfor7min.AllPCRreactionscontainedfinalconcentrationsof1µMofeachprimer,200µMdNTPs,1Xcresolredloadingdye,and1UoftheindicatedTaqpolymerase.SequencesforallPCRprimershavebeenpreviouslypublished(referencesavailable).DatacourtesyofDr.BenjaminAllen,Dept.Molecular&CellularBIOLOGy,HarvardUniversity.Figure4.PCRamplificationwasperformedunderstandardconditionsusingthreedifferentlotsofEconoTaqDNAPolymeraseandbuffer,orduplicatereactionswithTflDNApolymeraseandbuffer(Promega).Reactionscontainedprimersspecificforthe16SribosomalRNAgene,withBacillusgenomicDNA(+)ornoDNA(-)asatemplate(1450bpproductexpected).PleaseNote:SomeapplicationsinwhichLucigen'sEconoTaqDNAPolymerasecanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.ORDERINFORMATIONEconoTaqDNAPolymeraseisprovidedwithachoiceof10XReactionBufferwithMg++(“withMg++”);or10XReactionBufferwithoutMg++andaseparatetubeof25mMMgCl2(“separateMg++”).PleaseinquireforBulkpricing!
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。
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