产品说明
MaximizedpackagingefficiencyoflamBDa,cosmid,orfosmidDNAfortheconstructionoflargeinsertgenomiclibraries.ConstructgenomiclibrariesfrommethylatedorunmethylatedDNAwithequalefficiencyduetothelackofallknownrestrictionactivitiesintheextracts.Packagingefficienciesofupto3×109pfu/µgDNA.Simplifiedprotocoldoesnotrequirepremixingofdifferentextractcomponentsbeforeuse.MaxPlax™LambdaPackagingExtractsoffermaximuminvitropackagingefficienciesofcossite-containingmethylatedorunmethylatedDNA.TrADItionallambdapackagingextractsarederivedfromtwocomplementarylysogenicE.colistrains,BHB2690andBHB2688,asdescribedbyHohn.1MaxPlaxExtractsusearestriction-freeE.coliK-12strain,NM759,inplaceofstrainBHB2690.2ThesonicatedextractsofNM759arecombinedwithextractsofBHB2688toproduceMaxPlaxExtracts.EachlotofMaxPlaxExtractsistestedandguaranteedtomaintainapackagingefficiencyofgreaterthan1×109pfu/µgofControlLigatedLambdaDNAfor1yearfromthedateofpurchaseifstoredproperly.ACertificateofAnalysisstatingactualpackagingefficiency(pfu/µgDNA)isprovidedwitheachlotofMaxPlaxExtracts.Table1.PackagingofmethylatedandunmethylatedDNAusingMaxPlax™LambdaPackagingExtracts.CosmidInsertPfu/µgDNAUnmethylatedDNA3.5x107MethylatedDNA3.9x107ReferencesHohn,E.G.(1979)MethodsEnzymol.68,299.Gunther,E.G.etal.(1993)NucleicAcidsRes.21,3903.Fiandt,M.(2000)EpicentreForum7(3),14.ORDERINFORMATIONContents:PredispensedMaxPlax™Extracts,ControlLambdaDNA,ControlHostCells(E.coli).
美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
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